Cattle-Derived Microsatellite Markers to Study the Water Buffalo Genome

dc.contributor.authorStafuzza, Nedenia B. [UNESP]
dc.contributor.authorAmarala, M. Elisabete J. [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-12-03T13:11:06Z
dc.date.available2014-12-03T13:11:06Z
dc.date.issued2013-01-01
dc.description.abstractMicrosatellites are well-known DNA markers used in a variety of studies such as genome mapping, genetic diversity analysis, genetic conservation and phylogenetic studies. Although microsatellites are important markers, their development and characterization demands extensive time and high cost. Thus, before new markers are developed for a particular species, it is worthwhile to test the available markers from related species. In the present study, we evaluate cattle-derived microsatellite markers for genetic studies of water buffalo. Eighty-five percents of a total of 120 microsatellite markers were optimized using buffalo DNA (Bubalus bubalis). The results showed in this paper were also deposited in the National Center for Biological Information database (NCBI) (ProbeDB and UniSTS) for use in population genetic studies of buffalo by the scientific community. The use of heterologous primers significantly reduces the cost of developing specific markers for buffalo, providing a useful short cut for the genetic population analysis and gene mapping studies.en
dc.description.affiliationSao Paulo State Univ UNESP IBILCE, Dept Biol, BR-15054000 Sao Jose do Rio Preto, SP, Brazil
dc.description.affiliationUnespSao Paulo State Univ UNESP IBILCE, Dept Biol, BR-15054000 Sao Jose do Rio Preto, SP, Brazil
dc.format.extent681-685
dc.identifierhttp://ibic.lib.ku.ac.th/e-Bulletin/archive.htm
dc.identifier.citationBuffalo Bulletin. Bangkok: Int Buffalo Information Ctr, v. 32, p. 681-685, 2013.
dc.identifier.issn0125-6726
dc.identifier.urihttp://hdl.handle.net/11449/112861
dc.identifier.wosWOS:000331980300088
dc.language.isoeng
dc.publisherInt Buffalo Information Ctr
dc.relation.ispartofBuffalo Bulletin
dc.relation.ispartofjcr0.119
dc.relation.ispartofsjr0,217
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectBubalus bubalisen
dc.subjectMicrosatelliteen
dc.subjectPCR primersen
dc.subjectWater buffaloen
dc.titleCattle-Derived Microsatellite Markers to Study the Water Buffalo Genomeen
dc.typeArtigo
dcterms.rightsHolderInt Buffalo Information Ctr
unesp.author.orcid0000-0001-6432-2330[1]
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências Letras e Ciências Exatas, São José do Rio Pretopt

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