Optimization of an amperometric biosensor for the detection of hepatitis C virus using fractional factorial designs
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2008-01-01
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Soc Brasileira Quimica
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Fractional factorial design and factorial with center point design were applied to the development of an amperometric biosensor for the detection of the hepatitis C virus. Biomolecules were immobilized by adsorption on graphite electrodes modified with siloxane-poly(propyleneoxide) hybrid matrix prepared using the sol-gel method. Several parameters were optimized, such as the streptavidin concentration at 0.01 mg mL(-1) and 1.0% bovine serum albumin, the incubation time of the electrodes in the complementary DNA solution for 30 minutes and a 1: 1500 dilution of the avidin-peroxidase conjugate, among others. The application of chemometric studies has been efficient, since the best conditions have been established with a restricted number of experiments, indicating the influence of different factors on the system.
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Journal of The Brazilian Chemical Society. São Paulo: Soc Brasileira Quimica, v. 19, n. 4, p. 782-787, 2008.
