Validation of a new technique to detect Cryptosporidium spp. oocysts in bovine feces

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dc.contributor.authorInácio, Sandra Valéria [UNESP]
dc.contributor.authorGomes, Jancarlo Ferreira
dc.contributor.authorOliveira, Bruno César Miranda [UNESP]
dc.contributor.authorFalcão, Alexandre Xavier
dc.contributor.authorSuzuki, Celso Tetsuo Nagase
dc.contributor.authordos Santos, Bianca Martins
dc.contributor.authorde Aquino, Monally Conceição Costa [UNESP]
dc.contributor.authorde Paula Ribeiro, Rafaela Silva [UNESP]
dc.contributor.authorde Assunção, Danilla Mendes [UNESP]
dc.contributor.authorCasemiro, Pamella Almeida Freire [UNESP]
dc.contributor.authorMeireles, Marcelo Vasconcelos [UNESP]
dc.contributor.authorBresciani, Katia Denise Saraiva [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade Estadual de Campinas (UNICAMP)
dc.date.accessioned2018-12-11T17:06:10Z
dc.date.available2018-12-11T17:06:10Z
dc.date.issued2016-11-01
dc.description.abstractDue to its important zoonotic potential, cryptosporidiosis arouses strong interest in the scientific community, because, it was initially considered a rare and opportunistic disease. The parasitological diagnosis of the causative agent of this disease, the protozoan Cryptosporidium spp., requires the use of specific techniques of concentration and permanent staining, which are laborious and costly, and are difficult to use in routine laboratory tests. In view of the above, we conducted the feasibility, development, evaluation and intralaboratory validation of a new parasitological technique for analysis in optical microscopy of Cryptosporidium spp. oocysts, called TF-Test Coccidia, using fecal samples from calves from the city of Araçatuba, São Paulo. To confirm the aforementioned parasite and prove the diagnostic efficiency of the new technique, we used two established methodologies in the scientific literature: parasite concentration by centrifugal sedimentation and negative staining with malachite green (CSN-Malachite) and Nested-PCR. We observed good effectiveness of the TF-Test Coccidia technique, being statistically equivalent to CSN-Malachite. Thus, we verified the effectiveness of the TF-Test Coccidia parasitological technique for the detection of Cryptosporidium spp. oocysts and observed good concentration and morphology of the parasite, with a low amount of debris in the fecal smear.en
dc.description.affiliationUNESP − Universidade Estadual Paulista Júlio de Mesquita Filho Departamento de Apoio Produção e Saúde Animal Faculdade de Medicina Veterinária de Araçatuba, çatuba, São Paulo
dc.description.affiliationUNICAMP Universidade Estadual de Campinas Institutos de Biologia e Computação, Campinas
dc.description.affiliationUNICAMP Universidade Estadual de Campinas Instituto de Computação, Campinas
dc.description.affiliationUNICAMP Universidade Estadual de Campinas Instituto de Biologia, Campinas
dc.description.affiliationUnespUNESP − Universidade Estadual Paulista Júlio de Mesquita Filho Departamento de Apoio Produção e Saúde Animal Faculdade de Medicina Veterinária de Araçatuba, çatuba, São Paulo
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIdFAPESP: #99/06228-4
dc.format.extent1-5
dc.identifierhttp://dx.doi.org/10.1016/j.prevetmed.2016.09.020
dc.identifier.citationPreventive Veterinary Medicine, v. 134, p. 1-5.
dc.identifier.doi10.1016/j.prevetmed.2016.09.020
dc.identifier.file2-s2.0-84989225545.pdf
dc.identifier.issn0167-5877
dc.identifier.lattes5950594366829647
dc.identifier.lattes0903513897615274
dc.identifier.scopus2-s2.0-84989225545
dc.identifier.urihttp://hdl.handle.net/11449/173541
dc.language.isoeng
dc.relation.ispartofPreventive Veterinary Medicine
dc.relation.ispartofsjr1,144
dc.rights.accessRightsAcesso aberto
dc.sourceScopus
dc.subjectCoccidiosis
dc.subjectDiagnostic
dc.subjectParasites
dc.titleValidation of a new technique to detect Cryptosporidium spp. oocysts in bovine fecesen
dc.typeArtigo
unesp.author.lattes5950594366829647
unesp.author.lattes0903513897615274

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Artigos - Apoio, Produção e Saúde Animal - FMVA