Localization of 5-methylcytosine in metaphase chromosomes of diploid and triploid pacu fish, Piaractus mesopotamicus (Pisces, Characiformes)

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dc.contributor.authorde Almeida-Toledo, L. F.
dc.contributor.authorViegas-Pequignot, E.
dc.contributor.authorCoutinho-Barbosa, A. C.
dc.contributor.authorForesti, F.
dc.contributor.authorNiveleau, A.
dc.contributor.authorToledo-Filho, S. D.
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionHop Necker Enfants Malad
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionCNRS
dc.date.accessioned2014-05-20T13:51:17Z
dc.date.available2014-05-20T13:51:17Z
dc.date.issued1998-01-01
dc.description.abstractThe distribution of 5-methylcytosine (5-MeC) was investigated in fish chromosomes by indirect immunofluorescence using a highly specific 5-MeC monoclonal antibody. Diploid and artificially produced triploid specimens of the pacu fish, Piaractus mesopotamicus, were analyzed. The strong immunofluorescent signals were coincident with the heterochromatic regions of both diploids and triploids in a pattern that matched the C-banding pattern. In the euchromatin, heterogeneous labeling was observed along the chromatids. The weakness of this labeling hindered comparison of the fluorescence labeling of homologous chromosomes from diploid and triploid individuals. However, no striking differences were observed. The possibility that the euchromatin labeling by the 5-MeC antibody is related to the occurrence of mildly repetitive sequences in the genome of Piaractus is discussed.en
dc.description.affiliationUSP, Inst Biociencias, Dept Biol, BR-05422970 São Paulo, Brazil
dc.description.affiliationHop Necker Enfants Malad, INSERM, U383, Paris, France
dc.description.affiliationUNESP, Inst Biociencias, Dept Morfol, Botucatu, SP, Brazil
dc.description.affiliationCNRS, Inst Pasteur, Lyon, France
dc.description.affiliationUnespUNESP, Inst Biociencias, Dept Morfol, Botucatu, SP, Brazil
dc.format.extent21-24
dc.identifierhttp://dx.doi.org/10.1159/000015158
dc.identifier.citationCytogenetics and Cell Genetics. Basel: Karger, v. 83, n. 1-2, p. 21-24, 1998.
dc.identifier.doi10.1159/000015158
dc.identifier.issn0301-0171
dc.identifier.lattes0804793944846367
dc.identifier.urihttp://hdl.handle.net/11449/18311
dc.identifier.wosWOS:000078544000008
dc.language.isoeng
dc.publisherKarger
dc.relation.ispartofCytogenetics and Cell Genetics
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.titleLocalization of 5-methylcytosine in metaphase chromosomes of diploid and triploid pacu fish, Piaractus mesopotamicus (Pisces, Characiformes)en
dc.typeArtigo
dcterms.licensehttp://www.karger.com/Services/RightsPermissions
dcterms.rightsHolderKarger
unesp.author.lattes0804793944846367
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências, Botucatupt

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