Oxalate determination in urine using an immobilized enzyme on Sorghum vulgare seeds in a flow injection conductimetric system

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dc.contributor.authorOliveira Neto, Graciliano de
dc.contributor.authorTubino, Matthieu
dc.contributor.authorGodinho, Oswaldo Espirito Santo
dc.contributor.authorKubota, Lauro Tatsuo
dc.contributor.authorFernandes, João Roberto [UNESP]
dc.contributor.institutionUniversidade Estadual de Campinas (UNICAMP)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniv. Federal do Maranhão
dc.date.accessioned2014-05-27T11:18:18Z
dc.date.available2014-05-27T11:18:18Z
dc.date.issued1997-12-01
dc.description.abstractA flow-injection (FI) method was developed for the determination of oxalate in urine. It was based on the use of oxalate oxidase (E.C. 1.2.3.4) immobilized on ground seeds of the BR-303 Sorghum vulgare variety. A reactor was filled with this activated material, and the samples (200 μL) containing oxalate were passed through it, carried by a deionized water flow. The carbon dioxide produced by the enzyme reaction permeated through a microporous PTFE membrane, and was received in a water acceptor stream, promoting conductivity changes proportional to the oxalate concentration in the sample. The results obtained showed a useful linear range from 0.05 to 0.50 mmol dm-3. The proposed method, when compared with the Sigma enzymatic procedure, showed good correlation (Y = 0.006(±0.016) + 0.98(±0.019)X; r = 0.9995, Y = conductivity in μS, and X = concentration in mmol dm-3), selectivity, and sensitivity. The new immobilization approach promotes greater stability, allowing oxalate determination for 6 months. About 13 determinations can be performed per hour. The precision of the proposed method is about ± 3.2 % (r.s.d).en
dc.description.affiliationInst. de Química da UNICAMP C.P. 6154, 13083-970 Campinas - SP
dc.description.affiliationFaculdade de Ciências Depto de Química UNESP, Av. Luis Edmundo C. Coube s/n, 17033-360 Bauru - SP
dc.description.affiliationUniv. Federal do Maranhão, São Luís
dc.description.affiliationUnespFaculdade de Ciências Depto de Química UNESP, Av. Luis Edmundo C. Coube s/n, 17033-360 Bauru - SP
dc.format.extent47-51
dc.identifierhttp://dx.doi.org/10.1590/S0103-50531997000100009
dc.identifier.citationJournal of the Brazilian Chemical Society, v. 8, n. 1, p. 47-51, 1997.
dc.identifier.doi10.1590/S0103-50531997000100009
dc.identifier.file2-s2.0-3042869073.pdf
dc.identifier.issn0103-5053
dc.identifier.lattes8540599256820672
dc.identifier.scieloS0103-50531997000100009
dc.identifier.scopus2-s2.0-3042869073
dc.identifier.urihttp://hdl.handle.net/11449/65291
dc.identifier.wosWOS:A1997WP60200009
dc.language.isoeng
dc.relation.ispartofJournal of the Brazilian Chemical Society
dc.relation.ispartofjcr1.444
dc.relation.ispartofsjr0,357
dc.rights.accessRightsAcesso aberto
dc.sourceScopus
dc.subjectConductimetry
dc.subjectEnzyme immobilization
dc.subjectFlow injection
dc.subjectOxalate determination
dc.subjectOxalate oxidase
dc.subjectSorghum vulgare
dc.titleOxalate determination in urine using an immobilized enzyme on Sorghum vulgare seeds in a flow injection conductimetric systemen
dc.typeArtigo
dcterms.licensehttp://www.scielo.br/revistas/jbchs/iaboutj.htm
unesp.author.lattes8540599256820672
unesp.author.orcid0000-0001-8189-2618[4]
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Ciências, Baurupt
unesp.departmentQuímica - FCpt

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