The influence of violet LED application time on the esthetic efficacy and cytotoxicity of a 35% H2O2 bleaching gel

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dc.contributor.authorRibeiro, Rafael Antonio de Oliveira [UNESP]
dc.contributor.authorPeruchi, Victória [UNESP]
dc.contributor.authorFernandes, Lídia de Oliveira [UNESP]
dc.contributor.authorAnselmi, Caroline [UNESP]
dc.contributor.authorSoares, Igor Paulino Mendes [UNESP]
dc.contributor.authorHebling, Josimeri [UNESP]
dc.contributor.authorCosta, Carlos Alberto de Souza [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.date.accessioned2023-07-29T13:22:48Z
dc.date.available2023-07-29T13:22:48Z
dc.date.issued2022-12-01
dc.description.abstractObjective: To assess the potential influence of violet LED (V-LED) application time on the esthetic efficacy and cytotoxicity of a 35% H2O2 bleaching gel. Methodology: Stained and standardized enamel/dentin discs were subjected to one in-office tooth bleaching session (45 min), and the gel was either irradiated or not with V-LED. Thus, the following groups were established (n = 8): G1: No treatment (negative control, NC); G2: 35% H2O2 (positive control, PC); G3: 35%H2O2 + V-LED/15 min; G4: 35%H2O2 + V-LED/30 min; G5: 35%H2O2 + V-LED/45 min. First, esthetic efficacy was assessed (ΔE00 and ΔWI). Discs assembled in artificial pulp chambers were subjected to the same bleaching treatments. Then, the extracts (culture medium + diffused bleaching gel components) were collected and applied to MDPC-23 pulp cells, which were analyzed for viability (Live/Dead, MTT) and oxidative stress (OxS). The amount of H2O2 in the extracts was also determined (leuco crystal-violet/peroxidase). The data were subjected to ANOVA/Tukey at a 5% significance level. Results: Although esthetic efficacy did not differ among the irradiated groups (G3, G4, and G5) (p > 0.05), their results were higher than in G2 (PC; p < 0.05). In the irradiated groups, the cell viability and OxS as well as the amount of H2O2 in the extracts were statistically similar to G2 (PC), regardless of irradiation time (p > 0.05). Conclusion: Although V-LED improves the esthetic outcome of in-office tooth bleaching, increasing irradiation time does not effect the color changes and cytotoxicity of this professional therapy.en
dc.description.affiliationDepartment of Dental Materials and Prosthodontics School of Dentistry São Paulo State University (UNESP)
dc.description.affiliationDepartment of Restorative Dentistry School of Dentistry São Paulo State University (UNESP)
dc.description.affiliationDepartment of Morphology and Pediatric Dentistry School of Dentistry São Paulo State University (UNESP)
dc.description.affiliationDepartment of Physiology and Pathology School of Dentistry São Paulo State University (UNESP), Araraquara, Rua Humaitá, 1680
dc.description.affiliationUnespDepartment of Dental Materials and Prosthodontics School of Dentistry São Paulo State University (UNESP)
dc.description.affiliationUnespDepartment of Restorative Dentistry School of Dentistry São Paulo State University (UNESP)
dc.description.affiliationUnespDepartment of Morphology and Pediatric Dentistry School of Dentistry São Paulo State University (UNESP)
dc.description.affiliationUnespDepartment of Physiology and Pathology School of Dentistry São Paulo State University (UNESP), Araraquara, Rua Humaitá, 1680
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipIdFAPESP: 2020/09095-8
dc.description.sponsorshipIdFAPESP: 2021/01184-4
dc.description.sponsorshipIdCNPq: 302047/2019-0
dc.description.sponsorshipIdCNPq: 408721/2018-9
dc.identifierhttp://dx.doi.org/10.1016/j.pdpdt.2022.103069
dc.identifier.citationPhotodiagnosis and Photodynamic Therapy, v. 40.
dc.identifier.doi10.1016/j.pdpdt.2022.103069
dc.identifier.issn1873-1597
dc.identifier.issn1572-1000
dc.identifier.scopus2-s2.0-85138829832
dc.identifier.urihttp://hdl.handle.net/11449/247677
dc.language.isoeng
dc.relation.ispartofPhotodiagnosis and Photodynamic Therapy
dc.sourceScopus
dc.subjectCytotoxicity
dc.subjectOdontoblasts
dc.subjectTooth bleaching
dc.subjectViolet LED
dc.titleThe influence of violet LED application time on the esthetic efficacy and cytotoxicity of a 35% H2O2 bleaching gelen
dc.typeArtigo

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