Lipid content and apoptosis of in vitro-produced bovine embryos as determinants of susceptibility to vitrification

Sudano, Mateus Jose [UNESP]; Paschoal, Daniela Martins [UNESP]; Rascado, Tatiana da Silva [UNESP]; Ona Magalhaes, Luis Carlos [UNESP]; Crocomo, Leticia Ferrari [UNESP]; de Lima-Neto, Joao Ferreira [UNESP]; Landim-Alvarenga, Fernanda da Cruz [UNESP]

Lipid content and apoptosis of in vitro-produced bovine embryos as determinants of susceptibility to vitrification

Arquivos

WOS000289180900005.pdf (1.19 MB)

Data

2011-04-15

Autores

Sudano, Mateus Jose

Paschoal, Daniela Martins

Rascado, Tatiana da Silva

Ona Magalhaes, Luis Carlos

Crocomo, Leticia Ferrari

de Lima-Neto, Joao Ferreira

Landim-Alvarenga, Fernanda da Cruz

Editor

Elsevier B.V.

Tipo

Artigo

Direito de acesso

Acesso aberto

Resumo

The objective was to evaluate supplementation of fetal calf serum (FCS) and phenazine ethosulfate (PES), a metabolic regulator that inhibits fatty acid synthesis, in culture media during in vitro production (IVP) of bovine embryos. Taking oocyte fertilization (n = 4,320) as Day 0, four concentrations of FCS (0, 2.5, 5, and 10%) and three periods of exposure to PES (without addition-Control; after 60 h-PES Day 2.5 of embryo culture; and after 96 h-PES Day 4) were evaluated. Increasing FCS concentration in the culture media enhanced lipid accumulation (P < 0.05), increased apoptosis in fresh (2.5%: 19.1 +/- 1.8 vs 10%: 28.4 +/- 2.3, P < 0.05; mean +/- SEM) and vitrified (2.5%: 42.8 +/- 2.7 vs 10%: 69.2 +/- 3.4, P < 0.05) blastocysts, and reduced blastocoele re-expansion after vitrification (2.5%: 81.6 +/- 2.5 vs 10%: 67.3 +/- 3.5, P < 0.05). The addition of PES in culture media, either from Days 2.5 or 4, reduced lipid accumulation (P < 0.05) and increased blastocoele re-expansion after vitrification (Control: 72.0 +/- 3.0 vs PES Day 2.5: 79.9 +/- 2.8 or PES Day 4: 86.2 +/- 2.4, P < 0.05). However, just the use of PES from D4 reduced apoptosis in vitrified blastocysts (Control: 52.0 +/- 3.0 vs PES Day 4: 39.2 +/- 2.4, P < 0.05). Independent of FCS withdrawal or PES addition to culture media, the in vivo control group had lesser lipid accumulation, a lower apoptosis rate, and greater cryotolerance (P < 0.05). The increased lipid content was moderately correlated with apoptosis in vitrified blastocysts (r = 0.64, P = 0.01). In contrast, the increased apoptosis in fresh blastocysts was strongly correlated with apoptosis in vitrified blastocysts (r = 0.94, P < 0.0001). Therefore, using only 2.5% FCS and the addition of PES from Day 4, increased the survival of IVP embryos after vitrification. Moreover, embryo quality, represented by the fresh apoptosis rate, was better than lipid content for predicting embryo survival after vitrification. (C) 2011 Elsevier B.V. All rights reserved.