Peltate trichomes in the dormant shoot apex of Metrodorea nigra, a Rutaceae species with rhythmic growth

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dc.contributor.authorMachado, S. R. [UNESP]
dc.contributor.authorde Deus Bento, K. B. [UNESP]
dc.contributor.authorCanaveze, Y.
dc.contributor.authorRodrigues, T. M. [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.contributor.institutionFederal University of Rio de Janeiro
dc.date.accessioned2023-07-29T13:29:34Z
dc.date.available2023-07-29T13:29:34Z
dc.date.issued2023-01-01
dc.description.abstractIn Metrodorea nigra, a Rutaceae species with rhythmic growth, the shoot apex in the dormant stage is enclosed by modified stipules. The young organs are fully covered with peltate secretory trichomes, and these structures remain immersed in a hyaline exudate within a hood-shaped structure. Our study focused on the morpho-functional characterization of the peltate trichomes and cytological events associated with secretion. Shoot apices were collected during both dormant and active stages and processed for anatomical, cytochemical and ultrastructural studies. Trichomes initiate secretion early on, remain active throughout leaf development, but collapse as the leaves expand; at which time secretory cavities start differentiation in the mesophyll and secretion increases as the leaf reaches full expansion. The subcellular apparatus of the trichome head cells is consistent with hydrophilic and lipophilic secretion. Secretion involves two vesicle types: the smaller vesicles are PATAg-positive (periodic acid/thiocarbohydrazide/silver proteinate) for carbohydrates and the larger ones are PATAg-negative. In the first phase of secretory activity, the vesicles containing polysaccharides discharge their contents through exocytosis with the secretion accumulating beneath the cuticle, which detaches from the cell wall. Later, a massive discharge of lipophilic substances (lipids and terpenes/phenols) results in their accumulation between the wall and cuticle. Release of the secretions occurs throughout the cuticular microchannels. Continued protection of the leaves throughout shoot development is ensured by replacement of the collapsed secretory trichomes by oil-secreting cavities. Our findings provide new perspectives for understanding secretion regulation in shoot apices of woody species with rhythmic growth.en
dc.description.affiliationCenter of Electron Microscopy (CME) Institute of Biosciences of Botucatu (IBB) São Paulo State University (UNESP), SP
dc.description.affiliationPostgraduate Program in Plant Biology Interunits Paulo State University (UNESP), SP
dc.description.affiliationDepartment of Botany Institute of Biology Federal University of Rio de Janeiro, RJ
dc.description.affiliationDepartment of Biostatistics Plant Biology Parasitology and Zoology Institute of Biosciences – IBB São Paulo State University – UNESP, SP
dc.description.affiliationUnespCenter of Electron Microscopy (CME) Institute of Biosciences of Botucatu (IBB) São Paulo State University (UNESP), SP
dc.description.affiliationUnespPostgraduate Program in Plant Biology Interunits Paulo State University (UNESP), SP
dc.description.affiliationUnespDepartment of Biostatistics Plant Biology Parasitology and Zoology Institute of Biosciences – IBB São Paulo State University – UNESP, SP
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipIdCAPES: 001
dc.description.sponsorshipIdCNPq: 308982/2020-7
dc.description.sponsorshipIdCNPq: 312900/2021-0
dc.description.sponsorshipIdCNPq: 401053/2016-4
dc.format.extent161-175
dc.identifierhttp://dx.doi.org/10.1111/plb.13480
dc.identifier.citationPlant Biology, v. 25, n. 1, p. 161-175, 2023.
dc.identifier.doi10.1111/plb.13480
dc.identifier.issn1438-8677
dc.identifier.issn1435-8603
dc.identifier.scopus2-s2.0-85142275320
dc.identifier.urihttp://hdl.handle.net/11449/247920
dc.language.isoeng
dc.relation.ispartofPlant Biology
dc.sourceScopus
dc.subjectCytochemistry
dc.subjectcytology
dc.subjectpeltate secretory trichomes
dc.subjectRutaceae
dc.subjectultrastructure
dc.titlePeltate trichomes in the dormant shoot apex of Metrodorea nigra, a Rutaceae species with rhythmic growthen
dc.typeArtigo
unesp.author.orcid0000-0003-3137-8551[1]
unesp.author.orcid0000-0002-2208-911X[2]
unesp.author.orcid0000-0001-7516-9845[3]
unesp.author.orcid0000-0003-4993-101X[4]

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Botucatu - CME - Centro de Microscopia Eletrônica
Artigos - Parasitologia - IBB