Estimation of the metabolic rate by assessing carbon-13 turnover in broiler tissues using the stable isotope technique

Página do item simplificado
dc.contributor.authorPelícia, V. C. [UNESP]
dc.contributor.authorAraujo, P. C. [UNESP]
dc.contributor.authorLuiggi, F. G. [UNESP]
dc.contributor.authorStradiotti, A. C. [UNESP]
dc.contributor.authorDenadai, J. C. [UNESP]
dc.contributor.authorSartori, J. R. [UNESP]
dc.contributor.authorPacheco, P. D.G. [UNESP]
dc.contributor.authorDornelas, L. C. [UNESP]
dc.contributor.authorSilva, E. T. [UNESP]
dc.contributor.authorSouza-Kruliski, C. R. [UNESP]
dc.contributor.authorPimenta, G. E.M. [UNESP]
dc.contributor.authorDucatti, C. [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2018-12-11T17:17:51Z
dc.date.available2018-12-11T17:17:51Z
dc.date.issued2018-04-01
dc.description.abstractThe aim of this study was to estimate the metabolic rate of broiler tissues by assessing carbon turnover in different growth phases by using the stable isotope technique and by evaluating the natural changes in Carbon-13 (13C) between C3 and C4 cycle plants. Six-hundred 1-day-old broiler chicks were divided into six groups and subjected to dietary changes containing different isotopic values at 7, 14, 21, 28, and 35 days of age. Birds of Group 1 received diets composed predominantly of C3 cycle plants shortly after accommodation, while birds of other groups remained predominantly fed with C4 diet. The diet of Group 2 was mainly replaced by C3 diet on the 7th day of age. Group 3 started to receive C3 diet on the 14th day of age, whereas the diet of Group 4 was replaced on the 21st, Group 5 on the 28th. In Group 6, the substitution occurred on the 35th day of age. Samples of the pectoral muscle, keel, tibia, legs muscle, intestinal mucosa, blood, plasma, and feathers were collected. Carbon isotope ratios were determined by mass-spectrometry of isotope ratios. To measure the speed of carbon substitution after a set interval (turnover), a time exponential function was employed and obtained by exponential equations of the first order calculated with Minitab® 16 software. The carbon half-life varied from 1.78 to 8.20 days for the pectoral muscle, from 1.91 to 12.24 days for the keel, from 2.32 to 10.71 days for the tibia, from 1.87 to 9.43 days for the leg muscle, from 0.8 to 1.58 days for the intestinal mucosa, from 0.64 to 1.71 days for the plasma, from 2.61 to 11.07 days for blood, and from 1.84 to 28.41 days for the feathers during the total growth phase. With the exception of tibia and blood, all other tissues presented a high metabolic rate in the first week of the broilers’ life. The metabolic rate of tissues is faster in younger animals, and the metabolism slows down as the broilers get older. The metabolic rates in the pectoral muscle, keel, tibia, and feathers decreased with age, whereas those of the plasma and intestinal mucosa were high in all evaluated periods; thus, these tissues can be used to study traceability of feed at all stages of the broilers’ life.en
dc.description.affiliationCentro de Isótopos Estáveis Instituto de Biociências de Botucatu UNESP - Univ Estadual Paulista, Campus de Botucatu
dc.description.affiliationDepartamento de Melhoramento e Nutrição Animal Faculdade de Medicina Veterinária e Zootecnia UNESP – Univ Estadual Paulista, Campus de Botucatu
dc.description.affiliationUnespCentro de Isótopos Estáveis Instituto de Biociências de Botucatu UNESP - Univ Estadual Paulista, Campus de Botucatu
dc.description.affiliationUnespDepartamento de Melhoramento e Nutrição Animal Faculdade de Medicina Veterinária e Zootecnia UNESP – Univ Estadual Paulista, Campus de Botucatu
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIdFAPESP: 2008/57411-4
dc.description.sponsorshipIdFAPESP: 2011/17406-4
dc.format.extent8-14
dc.identifierhttp://dx.doi.org/10.1016/j.livsci.2018.01.019
dc.identifier.citationLivestock Science, v. 210, p. 8-14.
dc.identifier.doi10.1016/j.livsci.2018.01.019
dc.identifier.file2-s2.0-85041654391.pdf
dc.identifier.issn1871-1413
dc.identifier.scopus2-s2.0-85041654391
dc.identifier.urihttp://hdl.handle.net/11449/175848
dc.language.isoeng
dc.relation.ispartofLivestock Science
dc.relation.ispartofsjr0,730
dc.rights.accessRightsAcesso aberto
dc.sourceScopus
dc.subjectCarbon isotope
dc.subjectHalf-life
dc.subjectMetabolism
dc.subjectPoultry
dc.subjectTurnover
dc.titleEstimation of the metabolic rate by assessing carbon-13 turnover in broiler tissues using the stable isotope techniqueen
dc.typeArtigo

Arquivos

Pacote Original
Agora exibindo 1 - 1 de 1
Imagem de Miniatura
Nome:
2-s2.0-85041654391.pdf
Tamanho:
441.14 KB
Formato:
Adobe Portable Document Format
Descrição:
Baixar

Coleções

Botucatu - CIE - Centro de Isótopos Estáveis
Artigos - Melhoramento e Nutrição Animal - FMVZ