Immunosensor for diagnosis of Alzheimer disease using amyloid-β 1-40 peptide and silk fibroin thin films

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dc.contributor.authorGonçalves, J. M. [UNESP]
dc.contributor.authorLima, L. R. [UNESP]
dc.contributor.authorMoraes, M. L.
dc.contributor.authorRibeiro, S. J.L. [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2018-12-11T16:42:40Z
dc.date.available2018-12-11T16:42:40Z
dc.date.issued2016-11-01
dc.description.abstractLayer-by-Layer (LbL) films containing silk fibroin (SF) and the 40 aminoacid-long amyloid-β peptide (Aβ1-40) were prepared with the purpose of developing a new prototype of an electrochemical immunosensor. The film showed a satisfactory growth in quartz substrate and screen-printed carbon electrodes, as observed by UV-vis spectroscopy and cyclic voltammetric, respectively. The peptide immobilized in LbL films in junction with SF shows secondary structure induced, as shown by circular dichroism measurements, favoring the interaction SF/peptide LbL film with the specific antibody. Immunosensor showed a linear response in the presence of the antibody with concentrations from 0 to 10 ng mL- 1 both analyzed by current changes in 0.3 V and voltammogram area. This system can be applied as a new prototype for preliminary diagnosis of Alzheimer's disease.en
dc.description.affiliationInstituto de Química Universidade Estadual Paulista Júlio de Mesquita Filho UNESP, Rua Professor Francisco Degni, 55
dc.description.affiliationInstituto de Ciência e Tecnologia Universidade Federal de São Paulo UNIFESP, Rua Talim, 330
dc.description.affiliationUnespInstituto de Química Universidade Estadual Paulista Júlio de Mesquita Filho UNESP, Rua Professor Francisco Degni, 55
dc.format.extent338-342
dc.identifierhttp://dx.doi.org/10.1016/j.msec.2016.05.101
dc.identifier.citationMaterials Science and Engineering C, v. 68, p. 338-342.
dc.identifier.doi10.1016/j.msec.2016.05.101
dc.identifier.file2-s2.0-84973277831.pdf
dc.identifier.issn0928-4931
dc.identifier.scopus2-s2.0-84973277831
dc.identifier.urihttp://hdl.handle.net/11449/168712
dc.language.isoeng
dc.relation.ispartofMaterials Science and Engineering C
dc.relation.ispartofsjr1,110
dc.rights.accessRightsAcesso aberto
dc.sourceScopus
dc.subjectAlzheimer's disease
dc.subjectAmyloid-β peptide
dc.subjectImmunosensor
dc.subjectLayer-by-layer
dc.subjectSilk fibroin
dc.titleImmunosensor for diagnosis of Alzheimer disease using amyloid-β 1-40 peptide and silk fibroin thin filmsen
dc.typeArtigo
unesp.author.orcid0000-0003-0213-3686[3]

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