Lupeol, a pentacyclic triterpene, promotes migration, wound closure, and contractile effect in vitro: Possible involvement of PI3K/Akt and p38/ERK/MAPK pathways

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dc.contributor.authorBeserra, Fernando Pereira [UNESP]
dc.contributor.authorXue, Meilang
dc.contributor.authorDe Azevedo Maia, Gabriela Lemos
dc.contributor.authorRozza, Ariane Leite [UNESP]
dc.contributor.authorPellizzon, Cláudia Helena [UNESP]
dc.contributor.authorJackson, Christopher John
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionRoyal North Shore Hospital
dc.contributor.institutionFederal University of São Francisco Valley (UNIVASF)
dc.date.accessioned2019-10-06T16:54:16Z
dc.date.available2019-10-06T16:54:16Z
dc.date.issued2018-10-30
dc.description.abstractSkin wound healing is a dynamic and complex process involving several mediators at the cellular and molecular levels. Lupeol, a phytoconstituent belonging to the triterpenes class, is found in several fruit plants and medicinal plants that have been the object of study in the treatment of various diseases, including skin wounds. Various medicinal properties of lupeol have been reported in the literature, including anti-inflammatory, antioxidant, anti-diabetic, and anti-mutagenic effects. We investigated the effects of lupeol (0.1, 1, 10, and 20 µg/mL) on in vitro wound healing assays and signaling mechanisms in human neonatal foreskin keratinocytes and fibroblasts. Results showed that, at high concentrations, Lupeol reduced cell proliferation of both keratinocytes and fibroblasts, but increased in vitro wound healing in keratinocytes and promoted the contraction of dermal fibroblasts in the collagen gel matrix. This triterpene positively regulated matrix metalloproteinase (MMP)-2 and inhibited the NF-κB expression in keratinocytes, suggesting an anti-inflammatory effect. Lupeol also modulated the expression of keratin 16 according to the concentration tested. Additionally, in keratinocytes, lupeol treatment resulted in the activation of Akt, p38, and Tie-2, which are signaling proteins involved in cell proliferation and migration, angiogenesis, and tissue repair. These findings suggest that lupeol has therapeutic potential for accelerating wound healing.en
dc.description.affiliationDepartment of Morphology Institute of Biosciences São Paulo State University (UNESP)
dc.description.affiliationSutton Research Laboratory Kolling Institute of Medical Research University of Sydney Royal North Shore Hospital
dc.description.affiliationDepartment of Pharmacy Federal University of São Francisco Valley (UNIVASF)
dc.description.affiliationUnespDepartment of Morphology Institute of Biosciences São Paulo State University (UNESP)
dc.identifierhttp://dx.doi.org/10.3390/molecules23112819
dc.identifier.citationMolecules, v. 23, n. 11, 2018.
dc.identifier.doi10.3390/molecules23112819
dc.identifier.issn1420-3049
dc.identifier.lattes0019393779801069
dc.identifier.orcid0000-0002-4494-4180
dc.identifier.scopus2-s2.0-85055830274
dc.identifier.urihttp://hdl.handle.net/11449/189853
dc.language.isoeng
dc.relation.ispartofMolecules
dc.rights.accessRightsAcesso aberto
dc.sourceScopus
dc.subjectCell migration
dc.subjectFibroblasts
dc.subjectKeratinocytes
dc.subjectLupeol
dc.subjectWound healing
dc.titleLupeol, a pentacyclic triterpene, promotes migration, wound closure, and contractile effect in vitro: Possible involvement of PI3K/Akt and p38/ERK/MAPK pathwaysen
dc.typeArtigo
unesp.author.lattes2328604224911233[4]
unesp.author.lattes0019393779801069[5]
unesp.author.orcid0000-0002-4198-0135[4]
unesp.author.orcid0000-0002-4494-4180[5]

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