Proteomic Analysis of Gastrocnemius Muscle in Rats with Streptozotocin-Induced Diabetes and Chronically Exposed to Fluoride

dc.contributor.authorLeite, Aline Lima
dc.contributor.authorVaz Madureira Lobo, Janete Gualiume
dc.contributor.authorBarbosa da Silva Pereira, Heloisa Aparecida
dc.contributor.authorFernandes, Mileni Silva
dc.contributor.authorMartini, Tatiani
dc.contributor.authorZucki, Fernanda
dc.contributor.authorSumida, Doris Hissako [UNESP]
dc.contributor.authorRigalli, Alfredo
dc.contributor.authorRabelo Buzalaf, Marilia Afonso
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Federal de São Carlos (UFSCar)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionRosario Natl Univ
dc.description.abstractAdministration of high doses of fluoride (F) can alter glucose homeostasis and lead to insulin resistance (IR). This study determined the profile of protein expression in the gastrocnemius muscle of rats with streptozotocin-induced diabetes that were chronically exposed to F. Male Wistar rats (60 days old) were randomly distributed into two groups of 18 animals. In one group, diabetes was induced through the administration of streptozotocin. Each group (D-diabetic and ND-non-diabetic) was further divided into 3 subgroups each of which was exposed to a different F concentration via drinking water (0 ppm, 10 ppm or 50 ppm F, as NaF). After 22 days of treatment, the gastrocnemius muscle was collected and submitted to proteomic analysis (2D-PAGE followed by LC-MS/MS). Protein functions were classified by the GO biological process (ClueGO v2.0.7+Clupedia v1.0.8) and protein-protein interaction networks were constructed (PSICQUIC, Cytoscape). Quantitative intensity analysis of the proteomic data revealed differential expression of 75 spots for ND0 vs. D0, 76 for ND10 vs. D10, 58 spots for ND50 vs. D50, 52 spots for D0 vs. D10 and 38 spots for D0 vs. D50. The GO annotations with the most significant terms in the comparisons of ND0 vs. D0, ND10 vs. D10, ND50 vs. D50, D0 vs. D10 and D0 vs. D50, were muscle contraction, carbohydrate catabolic processes, generation of precursor metabolites and energy, NAD metabolic processes and gluconeogenesis, respectively. Analysis of subnetworks revealed that, in all comparisons, proteins with fold changes interacted with GLUT4. GLUT4 interacting proteins, such as MDH and the stress proteins HSPB8 and GRP78, exhibited decreased expression when D animals were exposed to F. The presence of the two stress proteins indicates an increase in IR, which might worsen diabetes. Future studies should evaluate whether diabetic animals treated with F have increased IR, as well as which molecular mechanisms are involved.en
dc.description.affiliationUniv Sao Paulo, Bauru Dent Sch, Dept Biol Sci, Bauru, SP, Brazil
dc.description.affiliationUniv Fed Sao Carlos, Ctr Biol Sci & Hlth, Dept Genet & Evolut, BR-13560 Sao Carlos, SP, Brazil
dc.description.affiliationUniv Estadual Paulista, UNESP, Aracatuba Dent Sch, Dept Basic Sci, Aracatuba, SP, Brazil
dc.description.affiliationRosario Natl Univ, Sch Med, Natl Sci & Tech Res Council CONICET, Bone Biol & Mineral Metab Lab, Rosario, Santa Fe, Argentina
dc.description.affiliationUnespUniv Estadual Paulista, UNESP, Aracatuba Dent Sch, Dept Basic Sci, Aracatuba, SP, Brazil
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIdFAPESP: 10/17736-1
dc.identifier.citationPlos One. San Francisco: Public Library Science, v. 9, n. 9, 10 p., 2014.
dc.publisherPublic Library Science
dc.relation.ispartofPlos One
dc.rights.accessRightsAcesso aberto
dc.sourceWeb of Science
dc.titleProteomic Analysis of Gastrocnemius Muscle in Rats with Streptozotocin-Induced Diabetes and Chronically Exposed to Fluorideen
dcterms.rightsHolderPublic Library Science
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Odontologia, Araçatubapt


Pacote Original
Agora exibindo 1 - 1 de 1
Imagem de Miniatura
993.23 KB
Adobe Portable Document Format