Molecular analysis reveals the diversity of Hepatozoon species naturally infecting domestic dogs in a northern region of Brazil

dc.contributor.authorGomes, Laise de Azevedo
dc.contributor.authorMoraes, Pablo Henrique Gonçalves
dc.contributor.authordo Nascimento, Luciana de Cássia Silva
dc.contributor.authorO'Dwyer, Lucia Helena [UNESP]
dc.contributor.authorNunes, Márcio Roberto Teixeira
dc.contributor.authorRossi, Adriana dos Reis Ponce
dc.contributor.authorAguiar, Délia Cristina Figueira
dc.contributor.authorGonçalves, Evonnildo Costa
dc.contributor.institutionUniversidade Federal do Pará (UFPA)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionInstituto Evandro Chagas
dc.date.accessioned2018-12-11T17:07:11Z
dc.date.available2018-12-11T17:07:11Z
dc.date.issued2016-10-01
dc.description.abstractThis study aimed to optimize molecular methods for detecting DNA of Hepatozoon spp. as well as identify the phylogenetic relationships of Hepatozoon strains naturally infecting domestic dogs in Belém, Pará, northern Brazil. Blood samples were collected from 138 dogs, and screened for Hepatozoon spp. using a new nested PCR assay. Positive samples were subjected to genetic characterization based on amplification and sequencing of approximately 670 bp of the Hepatozoon spp. 18S rRNA. Of the positive dogs, four shared the haplotype Belém 01, one dog presented the haplotype Belém 02 and two dogs shared the haplotype Belém 03. A Bayesian inference indicates that haplotypes Belém 01 and Belém 02 are phylogenetically related to H. canis, while Belém 03 is related to H. americanum. Overall, based on the first molecular evidence of H. americanum in Brazilian domestic dogs, the proposed protocol may improve the epidemiological investigation of canine hepatozoonosis.en
dc.description.affiliationLaboratório de Tecnologia Biomolecular Instituto de Ciências Biológicas Universidade Federal do Pará—UFPA, Belém
dc.description.affiliationInstituto de Biociências UNESP—Universidade Estadual Paulista Campus de Botucatu Departamento de Parasitologia, Distrito de Ribeirão Junior
dc.description.affiliationCentro de Inovações Tecnológicas Instituto Evandro Chagas, Levilândia
dc.description.affiliationUnespInstituto de Biociências UNESP—Universidade Estadual Paulista Campus de Botucatu Departamento de Parasitologia, Distrito de Ribeirão Junior
dc.format.extent1061-1066
dc.identifierhttp://dx.doi.org/10.1016/j.ttbdis.2016.09.008
dc.identifier.citationTicks and Tick-borne Diseases, v. 7, n. 6, p. 1061-1066, 2016.
dc.identifier.doi10.1016/j.ttbdis.2016.09.008
dc.identifier.file2-s2.0-84992195269.pdf
dc.identifier.issn1877-9603
dc.identifier.issn1877-959X
dc.identifier.scopus2-s2.0-84992195269
dc.identifier.urihttp://hdl.handle.net/11449/173670
dc.language.isoeng
dc.relation.ispartofTicks and Tick-borne Diseases
dc.relation.ispartofsjr1,421
dc.rights.accessRightsAcesso aberto
dc.sourceScopus
dc.subjectCanine hepatozoonosis
dc.subjectHepatozoon americanum
dc.subjectHepatozoon canis
dc.subjectMolecular epidemiology
dc.subjectNested PCR
dc.titleMolecular analysis reveals the diversity of Hepatozoon species naturally infecting domestic dogs in a northern region of Brazilen
dc.typeArtigo

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