Study of microbial interaction formed by Candida krusei and Candida glabrata: In vitro and In vivo studies

Rossoni, Rodnei Dennis [UNESP]; de Barros, Patrícia Pimentel [UNESP]; Freire, Fernanda [UNESP]; dos Santos, Jéssica Diane [UNESP]; Jorge, Antonio Olavo Cardoso [UNESP]; Junqueira, Juliana Campos [UNESP]

Study of microbial interaction formed by Candida krusei and Candida glabrata: In vitro and In vivo studies

Arquivos

S0103-64402017000600669.pdf (490.17 KB)

Data

2017-11-01

Autores

Rossoni, Rodnei Dennis

de Barros, Patrícia Pimentel

Freire, Fernanda

dos Santos, Jéssica Diane

Jorge, Antonio Olavo Cardoso

Junqueira, Juliana Campos

Tipo

Artigo

Direito de acesso

Acesso aberto

Resumo

Recently, the non-albicans Candida species have become recognized as an important source of infection and oral colonization by association of different species in a large number of immunosuppressed patients. The objective of this study was to evaluate the interactions between C. krusei and C. glabrata in biofilms formed in vitro and their ability to colonize the oral cavity of mouse model. Monospecies and mixed biofilms were developed of each strain, on 96-well microtiter plates for 48 h. These biofilms were analyzed by counting colony-forming units (CFU/mL) and by determining cell viability, using the XTT hydroxide colorimetric assay. For the in vivo study, twenty-four mice received topical applications of monospecie or mixed suspensions of each strain. After 48 h, yeasts were recovered from the mice and quantified by CFU/mL count. In the biofilm assays, the results for the CFU/mL count and the XTT assay showed that the two species studied were capable of forming high levels of in vitro monospecie biofilm. In mixed biofilm, the CFU of C. krusei increased (p=0.0001) and C. glabrata decreased (p=0.0001). The metabolic activity observed in XTT assay of mixed biofilm was significantly reduced compared with a single C. glabrata biofilm (p=0.0001). Agreeing with CFU in vitro count, C. glabrata CFU/mL values recovered from oral cavity of mice were statistically higher in the group with single infection (p=0.0001) than the group with mixed infection. We concluded that C. krusei inhibits C. glabrata and takes advantage to colonize the oral cavity and to form biofilms.