PCR associated with agar gel immunodiffusion assay improve caprine arthritis-encephalitis (CAEV) control

Nenhuma Miniatura disponível



Título da Revista

ISSN da Revista

Título de Volume


Elsevier B.V.


Caprine arthritis-encephalitis (CAE) is a multi-systemic viral syndrome in goats caused by small ruminant lentivirus (CAEV). The control measures prescribed for CAEV control are based on the identification of infected animals through a suitable serological test. The aim of this work was to improve the CAE control measures through the association of indirect (agar gel immunodiffusion-AGID) and direct (PCR) assays to CAEV diagnosis. Thirty-nine kids born to AGID-seropositive dairy goats were separated from their dams immediately after birth, fed heat-treated colostrum from AGID-seronegative goats and then after pasteurized goat milk. AGID was performed at birth before colostrum, at 9 and 12 months, and the AGID-seropositive animals were segregated. Seronegative goats were also submitted to PCR to detect proviral DNA in blood, and the positives were isolated. A study of accumulated residual negativity was performed using a 95% confidence limit rate. During the 12 month experimental period no clinical signs of CAEV were observed. At the end of that period 34 animals remained ACID-seronegative to CAEV, corresponding to 87% accumulated residual negativity. When submitted to PCR, 4 of the 34 ACID-seronegative animals showed positive results, leading to a 77% final accumulated negativity rate with 64-90% confidence limits. We conclude that the classical management practices recommended for CAEV control are insufficient in CAEV eradication programs and that PCR may be a useful tool for decreasing the risk of breeding AGID false negative animals (CAEV carriers). (C) 2008 Elsevier B.V. All rights reserved.



CAE, PCR, AGID, Diagnosis

Como citar

Small Ruminant Research. Amsterdam: Elsevier B.V., v. 81, n. 1, p. 18-20, 2009.