PCR associated with agar gel immunodiffusion assay improve caprine arthritis-encephalitis (CAEV) control

dc.contributor.authorModolo, J. R. [UNESP]
dc.contributor.authorStachissini, A. V. M. [UNESP]
dc.contributor.authorPadovani, C. R. [UNESP]
dc.contributor.authorAraujo Júnior, J. P. [UNESP]
dc.contributor.authorCastro, R. S.
dc.contributor.authorRavazzolo, A. P.
dc.contributor.authorLeite, B. L. S. [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade Federal Rural de Pernambuco (UFRPE)
dc.contributor.institutionUniversidade Federal do Rio Grande do Sul (UFRGS)
dc.date.accessioned2014-05-20T13:51:06Z
dc.date.available2014-05-20T13:51:06Z
dc.date.issued2009-01-01
dc.description.abstractCaprine arthritis-encephalitis (CAE) is a multi-systemic viral syndrome in goats caused by small ruminant lentivirus (CAEV). The control measures prescribed for CAEV control are based on the identification of infected animals through a suitable serological test. The aim of this work was to improve the CAE control measures through the association of indirect (agar gel immunodiffusion-AGID) and direct (PCR) assays to CAEV diagnosis. Thirty-nine kids born to AGID-seropositive dairy goats were separated from their dams immediately after birth, fed heat-treated colostrum from AGID-seronegative goats and then after pasteurized goat milk. AGID was performed at birth before colostrum, at 9 and 12 months, and the AGID-seropositive animals were segregated. Seronegative goats were also submitted to PCR to detect proviral DNA in blood, and the positives were isolated. A study of accumulated residual negativity was performed using a 95% confidence limit rate. During the 12 month experimental period no clinical signs of CAEV were observed. At the end of that period 34 animals remained ACID-seronegative to CAEV, corresponding to 87% accumulated residual negativity. When submitted to PCR, 4 of the 34 ACID-seronegative animals showed positive results, leading to a 77% final accumulated negativity rate with 64-90% confidence limits. We conclude that the classical management practices recommended for CAEV control are insufficient in CAEV eradication programs and that PCR may be a useful tool for decreasing the risk of breeding AGID false negative animals (CAEV carriers). (C) 2008 Elsevier B.V. All rights reserved.en
dc.description.affiliationUniv Estadual Paulista, Dept Saúde Anim & Vet Prevent, Fac Med Vet & Zootecnia, BR-18618000 Botucatu, SP, Brazil
dc.description.affiliationUniv Estadual Paulista, Inst Biociencias, BR-18618000 Botucatu, SP, Brazil
dc.description.affiliationUniversidade Federal Rural de Pernambuco (UFRPE), Dept Vet Med, Recife, PE, Brazil
dc.description.affiliationUniversidade Federal do Rio Grande do Sul (UFRGS), Fac Vet Porto Alegre, Porto Alegre, RS, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, Dept Saúde Anim & Vet Prevent, Fac Med Vet & Zootecnia, BR-18618000 Botucatu, SP, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, Inst Biociencias, BR-18618000 Botucatu, SP, Brazil
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipFundação de Amparo à Ciência e Tecnologia do Estado de Pernambuco (FACEPE)
dc.description.sponsorshipFinanciadora de Estudos e Projetos (FINEP)
dc.format.extent18-20
dc.identifierhttp://dx.doi.org/10.1016/j.smallrumres.2008.10.005
dc.identifier.citationSmall Ruminant Research. Amsterdam: Elsevier B.V., v. 81, n. 1, p. 18-20, 2009.
dc.identifier.doi10.1016/j.smallrumres.2008.10.005
dc.identifier.issn0921-4488
dc.identifier.lattes8727897080522289
dc.identifier.urihttp://hdl.handle.net/11449/18248
dc.identifier.wosWOS:000263204000004
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofSmall Ruminant Research
dc.relation.ispartofjcr0.974
dc.relation.ispartofsjr0,485
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectCAEen
dc.subjectPCRen
dc.subjectAGIDen
dc.subjectDiagnosisen
dc.titlePCR associated with agar gel immunodiffusion assay improve caprine arthritis-encephalitis (CAEV) controlen
dc.typeArtigo
dcterms.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dcterms.rightsHolderElsevier B.V.
unesp.author.lattes8727897080522289[3]
unesp.author.orcid0000-0002-7719-9682[3]
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências, Botucatupt

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