Generation of neural progenitor cells from porcine-induced pluripotent stem cells

dc.contributor.authorMachado, Lucas Simoes
dc.contributor.authorPieri, Naira Caroline Godoy
dc.contributor.authorBotigelli, Ramon Cesar [UNESP]
dc.contributor.authorCastro, Raquel Vasconcelos Guimaraes de [UNESP]
dc.contributor.authorSouza, Aline Fernanda de
dc.contributor.authorBridi, Alessandra
dc.contributor.authorLima, Marina Amaro
dc.contributor.authorFantinato Neto, Paulo
dc.contributor.authorPessoa, Lais Vicari de Figueiredo
dc.contributor.authorMartins, Simone Maria Massami Kitamura
dc.contributor.authorDe Andrade, Andre Furugen Cesar
dc.contributor.authorFreude, Kristine Karla
dc.contributor.authorBressan, Fabiana Fernandes
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniv Copenhagen
dc.date.accessioned2021-06-25T12:25:42Z
dc.date.available2021-06-25T12:25:42Z
dc.date.issued2020-11-23
dc.description.abstractIn this study, porcine embryonic fibroblasts (pEFs) were reprogrammed into porcine-induced pluripotent stem cells (piPSCs) using either human or mouse specific sequences for the OCT4, SOX2, c-Myc, and KLF4 transcription factors. In total, three pEFs lines were reprogrammed, cultured for at least 15 passages, and characterized regarding their pluripotency status (alkaline phosphatase expression, embryoid body formation, expression of exogenous and endogenous genes, and immunofluorescence). Two piPSC lines were further differentiated, using chemical inhibitors, into putative neural progenitor-like (NPC-like) cells with subsequent analyses of their morphology and expression of neural markers such as NESTIN and GFAP as well as immunofluorescent labeling of NESTIN, beta-TUBULIN III, and VIMENTIN. NPC-like cells were positive for all the neural markers tested. These results evidence of the generation of porcine NPC-like cells after in vitro induction with chemical inhibitors, representing an adequate model for future regenerative and translational medicine research.en
dc.description.affiliationUniv Sao Paulo, Fac Anim Sci & Food Engn, Dept Vet Med, Pirassununga, Brazil
dc.description.affiliationUniv Sao Paulo, Fac Vet Med & Anim Sci, Dept Surg, Postgrad Program Anat Domest & Wild Anim, Sao Paulo, Brazil
dc.description.affiliationUniv Sao Paulo, Fac Vet Med & Anim Sci, Dept Anim Reprod, Sao Paulo, Brazil
dc.description.affiliationSao Paulo State Univ, Inst Biosci, Dept Pharmacol, Botucatu, SP, Brazil
dc.description.affiliationSao Paulo State Univ, Fac Agr & Vet Sci, Dept Prevent Vet Med & Anim Reprod, Jaboticabal, Brazil
dc.description.affiliationUniv Sao Paulo, Fac Anim Sci & Food Engn, Dept Anim Sci, Pirassununga, Brazil
dc.description.affiliationUniv Copenhagen, Dept Vet & Anim Sci, Copenhagen, Denmark
dc.description.affiliationUnespSao Paulo State Univ, Inst Biosci, Dept Pharmacol, Botucatu, SP, Brazil
dc.description.affiliationUnespSao Paulo State Univ, Fac Agr & Vet Sci, Dept Prevent Vet Med & Anim Reprod, Jaboticabal, Brazil
dc.description.sponsorshipCoordination for the Improvement of Higher Education Personnel
dc.description.sponsorshipNational Council for Scientific and Technological Development
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIdNational Council for Scientific and Technological Development: 433133/2018-0
dc.description.sponsorshipIdFAPESP: 2013/08135-2
dc.description.sponsorshipIdFAPESP: 2015/26818-5
dc.description.sponsorshipIdFAPESP: 2017/02159-8
dc.description.sponsorshipIdFAPESP: 2017/13973-8
dc.format.extent1880-1891
dc.identifierhttp://dx.doi.org/10.1002/term.3143
dc.identifier.citationJournal Of Tissue Engineering And Regenerative Medicine. Hoboken: Wiley, v. 14, n. 12, p. 1880-1891, 2020.
dc.identifier.doi10.1002/term.3143
dc.identifier.issn1932-6254
dc.identifier.urihttp://hdl.handle.net/11449/209677
dc.identifier.wosWOS:000591386700001
dc.language.isoeng
dc.publisherWiley-Blackwell
dc.relation.ispartofJournal Of Tissue Engineering And Regenerative Medicine
dc.sourceWeb of Science
dc.subjectcellular reprogramming
dc.subjectinduced pluripotent stem cells (iPSC)
dc.subjectneural progenitor cells
dc.subjectswine
dc.titleGeneration of neural progenitor cells from porcine-induced pluripotent stem cellsen
dc.typeArtigo
dcterms.licensehttp://olabout.wiley.com/WileyCDA/Section/id-406071.html
dcterms.rightsHolderWiley-Blackwell
unesp.author.orcid0000-0001-9672-027X[4]
unesp.author.orcid0000-0001-9486-0121[6]
unesp.author.orcid0000-0002-3780-6046[9]
unesp.departmentMedicina Veterinária Preventiva e Reprodução Animal - FCAVpt

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