Landscape of transposable elements focusing on the B chromosome of the cichlid fish astatotilapia latifasciata

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dc.contributor.authorCoan, Rafael L. B. [UNESP]
dc.contributor.authorMartins, Cesar [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2018-12-11T17:37:16Z
dc.date.available2018-12-11T17:37:16Z
dc.date.issued2018-06-01
dc.description.abstractB chromosomes (Bs) are supernumerary elements found in many taxonomic groups. Most B chromosomes are rich in heterochromatin and composed of abundant repetitive sequences, especially transposable elements (TEs). B origin is generally linked to the A-chromosome complement (A). The first report of a B chromosome in African cichlids was in Astatotilapia latifasciata, which can harbor 0, 1, or 2 Bs Classical cytogenetic studies found high a TE content on this B chromosome. In this study, we aimed to understand TE composition and expression in the A. latifasciata genome and its relation to the B chromosome. We used bioinformatics analysis to explore the genomic organization of TEs and their composition on the B chromosome. The bioinformatics findings were validated by fluorescent in situ hybridization (FISH) and real-time PCR (qPCR). A. latifasciata has a TE content similar to that of other cichlid fishes and several expanded elements on its B chromosome. With RNA sequencing data (RNA-seq), we showed that all major TE classes are transcribed in the brain, muscle, and male and female gonads. An evaluation of TE transcription levels between B-and B+ individuals showed that few elements are differentially expressed between these groups and that the expanded B elements are not highly transcribed. Putative silencing mechanisms may act on the B chromosome of A. latifasciata to prevent the adverse consequences of repeat transcription and mobilization in the genome.en
dc.description.affiliationDepartment of Morphology Institute of Biosciences São Paulo State University (UNESP)
dc.description.affiliationUnespDepartment of Morphology Institute of Biosciences São Paulo State University (UNESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIdCNPq: 134446/2014-3
dc.description.sponsorshipIdFAPESP: 2013/04533-3
dc.description.sponsorshipIdFAPESP: 2014/16763-6
dc.description.sponsorshipIdFAPESP: 2015/16661-1
dc.description.sponsorshipIdCNPq: 305321/2015-3
dc.identifierhttp://dx.doi.org/10.3390/genes9060269
dc.identifier.citationGenes, v. 9, n. 6, 2018.
dc.identifier.doi10.3390/genes9060269
dc.identifier.file2-s2.0-85047921684.pdf
dc.identifier.issn2073-4425
dc.identifier.lattes8858800699425352
dc.identifier.orcid0000-0003-3534-974X
dc.identifier.scopus2-s2.0-85047921684
dc.identifier.urihttp://hdl.handle.net/11449/179914
dc.language.isoeng
dc.relation.ispartofGenes
dc.relation.ispartofsjr1,820
dc.rights.accessRightsAcesso aberto
dc.sourceScopus
dc.subjectCytogenetics
dc.subjectEvolution
dc.subjectExtra chromosomes
dc.subjectGenomics
dc.subjectRepetitive elements
dc.subjectRNA-Seq
dc.subjectSupernumerary elements
dc.titleLandscape of transposable elements focusing on the B chromosome of the cichlid fish astatotilapia latifasciataen
dc.typeArtigo
unesp.author.lattes8858800699425352[2]
unesp.author.orcid0000-0003-3534-974X[2]

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Artigos - Morfologia - IBB