Inhibitory effect of probiotic Lactobacillus supernatants from the oral cavity on Streptococcus mutans biofilms

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dc.contributor.authorRossoni, Rodnei Dennis [UNESP]
dc.contributor.authorVelloso, Marisol dos Santos [UNESP]
dc.contributor.authorde Barros, Patrícia Pimentel [UNESP]
dc.contributor.authorde Alvarenga, Janaina Araújo [UNESP]
dc.contributor.authorSantos, Jéssica Diane dos [UNESP]
dc.contributor.authorSantos Prado, Ana Carolina Chipoletti dos [UNESP]
dc.contributor.authorRibeiro, Felipe de Camargo [UNESP]
dc.contributor.authorAnbinder, Ana Lia [UNESP]
dc.contributor.authorJunqueira, Juliana Campos [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2018-12-11T17:21:51Z
dc.date.available2018-12-11T17:21:51Z
dc.date.issued2018-10-01
dc.description.abstractProbiotics can release bioactive substances that can inhibit the growth and biofilm formation of pathogenic microorganisms such as Streptococcus mutans. In this context, we evaluated whether the supernatants of Lactobacillus strains isolated from caries-free subjects can inhibit S. mutans, one of the most important bacteria for dental caries. First, the supernatants of 22 Lactobacillus strains were screened for antibacterial activity against S. mutans in planktonic cultures. All 22 Lactobacillus strains studied (100%) showed antibacterial activity. Thereafter, the Lactobacillus strains with the greatest reductions in the planktonic S. mutans cultures were tested on biofilms. The L. fermentum 20.4, L. paracasei 11.6, L. paracasei 20.3 and L. paracasei 25.4 strains could significantly reduce the number of S. mutans cells in biofilms formed in hydroxyapatite (p < 0.05). This reduction was also confirmed by scanning electron microscopy analysis and was not caused by the decreased pH value in the medium (p > 0.05). In addition, the supernatants of these probiotic strains could also reduce the total biomass of S. mutans biofilms (p < 0.05). In conclusion, most of the Lactobacillus strains tested have some antibacterial activity against S. mutans. L. fermentum 20.4, L. paracasei 11.6, L. paracasei 20.3 and L. paracasei 25.4 produce bioactive substances that caused a significant reduction in S. mutans biofilms.en
dc.description.affiliationDepartment of Biosciences and Oral Diagnosis São Paulo State University (Unesp) Institute of Science and Technology, São José dos Campos
dc.description.affiliationUnespDepartment of Biosciences and Oral Diagnosis São Paulo State University (Unesp) Institute of Science and Technology, São José dos Campos
dc.format.extent361-367
dc.identifierhttp://dx.doi.org/10.1016/j.micpath.2018.07.032
dc.identifier.citationMicrobial Pathogenesis, v. 123, p. 361-367.
dc.identifier.doi10.1016/j.micpath.2018.07.032
dc.identifier.file2-s2.0-85050507566.pdf
dc.identifier.issn1096-1208
dc.identifier.issn0882-4010
dc.identifier.scopus2-s2.0-85050507566
dc.identifier.urihttp://hdl.handle.net/11449/176638
dc.language.isoeng
dc.relation.ispartofMicrobial Pathogenesis
dc.relation.ispartofsjr0,751
dc.relation.ispartofsjr0,751
dc.rights.accessRightsAcesso aberto
dc.sourceScopus
dc.subjectBiofilm
dc.subjectLactobacillus
dc.subjectOral pathogens
dc.subjectStreptococcus mutans
dc.subjectSupernatant
dc.titleInhibitory effect of probiotic Lactobacillus supernatants from the oral cavity on Streptococcus mutans biofilmsen
dc.typeArtigo
unesp.author.lattes6097967943008273[8]
unesp.author.orcid0000-0003-3930-4274[8]

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