Postharvest harpin or Bacillus thuringiensis treatments suppress citrus black spot in 'Valencia' oranges

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dc.contributor.authorLucon, C. M. M.
dc.contributor.authorGuzzo, S. D.
dc.contributor.authorde Jesus, C. O.
dc.contributor.authorPascholati, S. F.
dc.contributor.authorde Goes, A. [UNESP]
dc.contributor.institutionCtr Pesquisa & Desenvolvimento Sanidade Vegetal
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-20T13:14:49Z
dc.date.available2014-05-20T13:14:49Z
dc.date.issued2010-07-01
dc.description.abstractCitrus black spot (CBS) caused by Guignardia citricarpa represents an important threat to citriculture in Brazil. Limited information is available regarding potential biological control agents and new alternative compounds that may provide protection of orange fruits against the disease. In this study, the effects of commercial products based on Bacillus thuringiensis var. kurstaki (Bt) bacterium, Bt pure isolates and Harpin protein (Messenger (R)) on the postharvest control of CBS, were evaluated in 'Valencia' sweet orange fruits harvested for three consecutive years in a citrus grove. The fruits were sprayed with the following products: DiPel (R) WP (Bt, subspecies, kurstaki strain HD-1,16,000 International Units mg(-1), 32 g active ingredient kg(-1)) (1, 20 and 50 mg ml(-1)), Dimy Pel (R) WP (Bt, subspecies, kurstaki, strain HD-1, 17,600 IU mg(-1), 26 g active ingredient l(-1)) (2, 20 and 50 mg ml(-1)), Messenger (R) (3% harpin protein) (1 and 2 mg ml(-1)) and fungicide Tecto (R) Flowable SC (thiabendazole, 485 gl(-1)) (0.8g active ingredient l(-1)), besides the Bt isolates, Bt- HD-567, Bt- DiPel and Bt- Dimy (9 x 10(8) CFU ml(-1)). Ten days after treatment, the number of newly developed CBS lesions and pycnidia produced were evaluated using fifty fruits per treatment. The Dimy Pel (R) and Messenger (R) reduced the number of new developed CBS lesions on fruits in up to 67% and 62%, respectively. All applied treatments drastically decreased the number of pycnidia produced in the CBS lesions on orange fruits with 85% to 96% reductions compared to the untreated control. Volatile compounds produced by the isolates Bt- HD-567, Bt- Dimy and Bt- DiPel, reduced the number of lesions on treated fruits by 70%, 65% and 71% compared to the control, respectively. In addition, the survival of Bt isolates on orange fruit surfaces were evaluated by recovering and quantifying the number of CFU every seven days for up to 28 days. The declines in survival rates on orange fruit surfaces were drastic for the three strains of Bt in the first week. The CFU numbers of all applied isolates declined by 4 to 5 orders of magnitude after storage at room temperature for 28 days. In vitro assays revealed that the Bt isolates significantly reduced the mycelial growth of the pathogen, ranging from 32% to 51%, compared to the control, whereas no inhibitory effect was observed in the presence of Messenger (R). (C) 2010 Elsevier Ltd. All rights reserved.en
dc.description.affiliationCtr Pesquisa & Desenvolvimento Sanidade Vegetal, Lab Bioquim Fitopatol, Inst Biol, BR-04010970 São Paulo, Brazil
dc.description.affiliationUniv São Paulo, ESALQ, Dept Fitopatol, BR-13400970 Piracicaba, SP, Brazil
dc.description.affiliationUniv Estadual Paulista, FCAV, Dept Fitossanidade, BR-14884900 Jaboticabal, SP, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, FCAV, Dept Fitossanidade, BR-14884900 Jaboticabal, SP, Brazil
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIdFAPESP: 01/10993-0
dc.format.extent766-772
dc.identifierhttp://dx.doi.org/10.1016/j.cropro.2010.02.018
dc.identifier.citationCrop Protection. Oxford: Elsevier B.V., v. 29, n. 7, p. 766-772, 2010.
dc.identifier.doi10.1016/j.cropro.2010.02.018
dc.identifier.issn0261-2194
dc.identifier.urihttp://hdl.handle.net/11449/2163
dc.identifier.wosWOS:000279365500019
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofCrop Protection
dc.relation.ispartofjcr1.920
dc.relation.ispartofsjr0,820
dc.rights.accessRightsAcesso aberto
dc.sourceWeb of Science
dc.subjectPlant-pathogen interactionen
dc.subjectInduced resistanceen
dc.subjectBiological controlen
dc.subjectPlant defense mechanismsen
dc.titlePostharvest harpin or Bacillus thuringiensis treatments suppress citrus black spot in 'Valencia' orangesen
dc.typeCarta
dcterms.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dcterms.rightsHolderElsevier B.V.
unesp.author.lattes0786750280869349[5]
unesp.author.orcid0000-0002-7749-3523[5]
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Ciências Agrárias e Veterinárias, Jaboticabalpt

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