Association of hyaluronic acid with a deproteinized bovine graft improves bone repair and increases bone formation in critical-size bone defects

dc.contributor.authorMatheus, Henrique R [UNESP]
dc.contributor.authorErvolino, Edilson [UNESP]
dc.contributor.authorGusman, David Jonathan Rodrigues [UNESP]
dc.contributor.authorAlves, Breno Edson Sendão [UNESP]
dc.contributor.authorFiorin, Luiz Guilherme [UNESP]
dc.contributor.authorPereira, Priscilla Aparecida
dc.contributor.authorde Almeida, Juliano Milanezi [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionPrivate practice. Director of the Pro-clinic Nucleus of Orofacial Harmonization
dc.date.accessioned2021-06-25T11:09:07Z
dc.date.available2021-06-25T11:09:07Z
dc.date.issued2020-01-01
dc.description.abstractBackground: This study is designed to evaluate the potential of different formulations of hyaluronic acid (HA) to improve new bone formation in critical-size calvaria defect (CSD) when combined with a deproteinized bovine graft (DBG) material. Methods: Thirty male rats were used. A 5-mm-diameter CSD was created and three experimental groups (n = 10) were randomly assigned based on the treatments performed. Group DBG: CSD filled with a DBG; group DBG/LV: CSD filled by the combination of DBG and HA in a low-viscosity crosslinking agent; group DBG/HV: CSD filled by the combination of DBG and HA in a high-viscosity crosslinking agent. Animals were euthanized 30 days postoperatively. Histological, histometric (percentage of newly formed bone [PNFB], percentage of remaining graft particles, histochemical, and immunohistochemical (bone morphogenetic protein 2/4 [BMP2/4], osteocalcin [OCN], and tartrate-resistant acid phosphatase [TRAP]) analyses were performed. Results: The highest PNFB was observed in DBG/HV when compared with the other groups (P ≤0.05). DBG/LV and DBG/HV presented almost no inflammatory cells. In contrast, inflammation was observed in group DBG. Extensive resorption of graft particles was observed in group DBG, which was not present in DBG/LV and DBG/HV as confirmed by the larger size of the particles (P ≤0.05). BMP2/4 and OCN immunolabeling were higher in DBG/HV when compared with group DBG (P ≤0.05). Increased number of TRAP-positive cells was observed in DBG/LV and DBG/HV (P ≤0.05). Lower percentage of mature collagen fibers was observed in DBG/HV (P ≤0.05). Conclusion: The combination of HA in a high-viscosity crosslinking agent with DBG improves the bone repair process and increases the amount of newly formed bone towards CSDs in rat calvaria.en
dc.description.affiliationDepartment of Diagnosis and Surgery‒Periodontics Division School of Dentistry São Paulo State University (UNESP)
dc.description.affiliationDepartment of Basic Science School of Dentistry São Paulo State University (UNESP)
dc.description.affiliationPrivate practice. Director of the Pro-clinic Nucleus of Orofacial Harmonization
dc.description.affiliationUnespDepartment of Diagnosis and Surgery‒Periodontics Division School of Dentistry São Paulo State University (UNESP)
dc.description.affiliationUnespDepartment of Basic Science School of Dentistry São Paulo State University (UNESP)
dc.identifierhttp://dx.doi.org/10.1002/JPER.20-0613
dc.identifier.citationJournal of Periodontology.
dc.identifier.doi10.1002/JPER.20-0613
dc.identifier.issn0022-3492
dc.identifier.scopus2-s2.0-85097763783
dc.identifier.urihttp://hdl.handle.net/11449/208252
dc.language.isoeng
dc.relation.ispartofJournal of Periodontology
dc.sourceScopus
dc.subjectanimal experimentation
dc.subjectbiomarkers
dc.subjectbone
dc.subjectbone regeneration
dc.subjectbone transplantation
dc.titleAssociation of hyaluronic acid with a deproteinized bovine graft improves bone repair and increases bone formation in critical-size bone defectsen
dc.typeArtigo
unesp.author.orcid0000-0003-3318-5980[1]
unesp.author.orcid0000-0002-5995-5747[7]

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