Post-thaw viability of in vivo-produced canine blastocysts cryopreserved by slow freezing
dc.contributor.author | Guaitolini, C. Renato de Freitas [UNESP] | |
dc.contributor.author | Taffarel, M. Onghero [UNESP] | |
dc.contributor.author | Teixeira, N. Soares | |
dc.contributor.author | Sudano, M. Jose [UNESP] | |
dc.contributor.author | Freitas, P. Maria Coletto | |
dc.contributor.author | Lopes, Maria Denise [UNESP] | |
dc.contributor.author | Landim-Alvarenga, Fernanda da Cruz [UNESP] | |
dc.contributor.author | Oliveira, C. Alvarenga de | |
dc.contributor.author | Luz, M. Rezende | |
dc.contributor.institution | Universidade Federal do Espírito Santo (UFES) | |
dc.contributor.institution | Universidade Estadual Paulista (Unesp) | |
dc.contributor.institution | Universidade Federal de Minas Gerais (UFMG) | |
dc.contributor.institution | Universidade de São Paulo (USP) | |
dc.date.accessioned | 2013-09-30T18:29:07Z | |
dc.date.accessioned | 2014-05-20T13:42:18Z | |
dc.date.available | 2013-09-30T18:29:07Z | |
dc.date.available | 2014-05-20T13:42:18Z | |
dc.date.issued | 2012-08-01 | |
dc.description.abstract | The objectives were to evaluate the reexpansion blastocoele rate, post-thaw viability, and in vitro development of canine blastocysts cryopreserved by slow freezing in 1.0 m glycerol (GLY) or 1.5 m ethylene glycol (EG). Fifty-one in vivo-produced canine blastocysts were randomly allocated in two groups: GLY (n = 26) and EG (n = 25). After thawing, embryos from MO were immediately stained with the fluorescent probes propidium iodide and Hoechst 33 342 to evaluate cellular viability. Frozen-thawed embryos from M3 and M6 were cultured in SOFaa medium + 10% FCS at 38.5 degrees C under an atmosphere of 5% CO2 with maximum humidity, for 3 and 6 days, respectively, and similarly stained. The blastocoele reexpansion rate (24 h after in vitro culture) did not differ between GLY (76.5%) and EG (68.8%). Post-thaw viable cells rate were not significantly different between GLY and EG (66.5 +/- 4.8 and 57.3 +/- 4.8, respectively, mean +/- SEM), or among MO (62.3 +/- 5.7%), M3 (56.9 +/- 6.0%), and M6 (66.5 +/- 6.0%). In conclusion, canine blastocysts cryopreserved by slow freezing in 1.0 m glycerol or 1.5 m ethylene glycol, had satisfactory blastocoele reexpansion rates, similar post-thawing viability, and remained viable for up to 6 days of in vitro culture. (C) 2012 Elsevier B.V. All rights reserved. | en |
dc.description.affiliation | Universidade Federal do Espírito Santo (UFES), Lab Anim Reprod, Dept Vet Med, BR-29500000 Alegre, ES, Brazil | |
dc.description.affiliation | São Paulo State Univ, UNESP, Sch Vet Med & Anim Sci, FMVZ,Dept Anim Reprod & Vet Radiol, BR-18618970 Botucatu, SP, Brazil | |
dc.description.affiliation | Universidade Federal de Minas Gerais (UFMG), Sch Vet, Dept Vet Clin & Surg, Sect Anim Reprod, BR-30123970 Belo Horizonte, MG, Brazil | |
dc.description.affiliation | Univ São Paulo, Lab Hormonal Assays, Dept Anim Reprod, Fac Vet Med & Anim Sci, BR-05508270 São Paulo, Brazil | |
dc.description.affiliationUnesp | São Paulo State Univ, UNESP, Sch Vet Med & Anim Sci, FMVZ,Dept Anim Reprod & Vet Radiol, BR-18618970 Botucatu, SP, Brazil | |
dc.description.sponsorship | Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) | |
dc.description.sponsorship | Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) | |
dc.description.sponsorshipId | CNPq: 36600736/2007 | |
dc.format.extent | 576-582 | |
dc.identifier | http://dx.doi.org/10.1016/j.theriogenology.2012.03.003 | |
dc.identifier.citation | Theriogenology. New York: Elsevier B.V., v. 78, n. 3, p. 576-582, 2012. | |
dc.identifier.doi | 10.1016/j.theriogenology.2012.03.003 | |
dc.identifier.file | WOS000306538100011.pdf | |
dc.identifier.issn | 0093-691X | |
dc.identifier.lattes | 6666129914663018 | |
dc.identifier.uri | http://hdl.handle.net/11449/14702 | |
dc.identifier.wos | WOS:000306538100011 | |
dc.language.iso | eng | |
dc.publisher | Elsevier B.V. | |
dc.relation.ispartof | Theriogenology | |
dc.relation.ispartofjcr | 2.136 | |
dc.rights.accessRights | Acesso aberto | |
dc.source | Web of Science | |
dc.subject | Cryopreservation | en |
dc.subject | Embryo viability | en |
dc.subject | Blastocoele reexpansion | en |
dc.subject | Glycerol | en |
dc.subject | Ethylene glycol | en |
dc.subject | Dog | en |
dc.title | Post-thaw viability of in vivo-produced canine blastocysts cryopreserved by slow freezing | en |
dc.type | Artigo | |
dcterms.license | http://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy | |
dcterms.rightsHolder | Elsevier B.V. | |
unesp.author.lattes | 6666129914663018 | |
unesp.author.orcid | 0000-0002-6643-3461[2] | |
unesp.campus | Universidade Estadual Paulista (Unesp), Faculdade de Medicina Veterinária e Zootecnia, Botucatu | pt |
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