Post-thaw viability of in vivo-produced canine blastocysts cryopreserved by slow freezing

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dc.contributor.authorGuaitolini, C. Renato de Freitas [UNESP]
dc.contributor.authorTaffarel, M. Onghero [UNESP]
dc.contributor.authorTeixeira, N. Soares
dc.contributor.authorSudano, M. Jose [UNESP]
dc.contributor.authorFreitas, P. Maria Coletto
dc.contributor.authorLopes, Maria Denise [UNESP]
dc.contributor.authorLandim-Alvarenga, Fernanda da Cruz [UNESP]
dc.contributor.authorOliveira, C. Alvarenga de
dc.contributor.authorLuz, M. Rezende
dc.contributor.institutionUniversidade Federal do Espírito Santo (UFES)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade Federal de Minas Gerais (UFMG)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.date.accessioned2013-09-30T18:29:07Z
dc.date.accessioned2014-05-20T13:42:18Z
dc.date.available2013-09-30T18:29:07Z
dc.date.available2014-05-20T13:42:18Z
dc.date.issued2012-08-01
dc.description.abstractThe objectives were to evaluate the reexpansion blastocoele rate, post-thaw viability, and in vitro development of canine blastocysts cryopreserved by slow freezing in 1.0 m glycerol (GLY) or 1.5 m ethylene glycol (EG). Fifty-one in vivo-produced canine blastocysts were randomly allocated in two groups: GLY (n = 26) and EG (n = 25). After thawing, embryos from MO were immediately stained with the fluorescent probes propidium iodide and Hoechst 33 342 to evaluate cellular viability. Frozen-thawed embryos from M3 and M6 were cultured in SOFaa medium + 10% FCS at 38.5 degrees C under an atmosphere of 5% CO2 with maximum humidity, for 3 and 6 days, respectively, and similarly stained. The blastocoele reexpansion rate (24 h after in vitro culture) did not differ between GLY (76.5%) and EG (68.8%). Post-thaw viable cells rate were not significantly different between GLY and EG (66.5 +/- 4.8 and 57.3 +/- 4.8, respectively, mean +/- SEM), or among MO (62.3 +/- 5.7%), M3 (56.9 +/- 6.0%), and M6 (66.5 +/- 6.0%). In conclusion, canine blastocysts cryopreserved by slow freezing in 1.0 m glycerol or 1.5 m ethylene glycol, had satisfactory blastocoele reexpansion rates, similar post-thawing viability, and remained viable for up to 6 days of in vitro culture. (C) 2012 Elsevier B.V. All rights reserved.en
dc.description.affiliationUniversidade Federal do Espírito Santo (UFES), Lab Anim Reprod, Dept Vet Med, BR-29500000 Alegre, ES, Brazil
dc.description.affiliationSão Paulo State Univ, UNESP, Sch Vet Med & Anim Sci, FMVZ,Dept Anim Reprod & Vet Radiol, BR-18618970 Botucatu, SP, Brazil
dc.description.affiliationUniversidade Federal de Minas Gerais (UFMG), Sch Vet, Dept Vet Clin & Surg, Sect Anim Reprod, BR-30123970 Belo Horizonte, MG, Brazil
dc.description.affiliationUniv São Paulo, Lab Hormonal Assays, Dept Anim Reprod, Fac Vet Med & Anim Sci, BR-05508270 São Paulo, Brazil
dc.description.affiliationUnespSão Paulo State Univ, UNESP, Sch Vet Med & Anim Sci, FMVZ,Dept Anim Reprod & Vet Radiol, BR-18618970 Botucatu, SP, Brazil
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipIdCNPq: 36600736/2007
dc.format.extent576-582
dc.identifierhttp://dx.doi.org/10.1016/j.theriogenology.2012.03.003
dc.identifier.citationTheriogenology. New York: Elsevier B.V., v. 78, n. 3, p. 576-582, 2012.
dc.identifier.doi10.1016/j.theriogenology.2012.03.003
dc.identifier.fileWOS000306538100011.pdf
dc.identifier.issn0093-691X
dc.identifier.lattes6666129914663018
dc.identifier.urihttp://hdl.handle.net/11449/14702
dc.identifier.wosWOS:000306538100011
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofTheriogenology
dc.relation.ispartofjcr2.136
dc.rights.accessRightsAcesso aberto
dc.sourceWeb of Science
dc.subjectCryopreservationen
dc.subjectEmbryo viabilityen
dc.subjectBlastocoele reexpansionen
dc.subjectGlycerolen
dc.subjectEthylene glycolen
dc.subjectDogen
dc.titlePost-thaw viability of in vivo-produced canine blastocysts cryopreserved by slow freezingen
dc.typeArtigo
dcterms.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dcterms.rightsHolderElsevier B.V.
unesp.author.lattes6666129914663018
unesp.author.orcid0000-0002-6643-3461[2]
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Medicina Veterinária e Zootecnia, Botucatupt

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