Evaluation of an experimental rat model for comparative studies of bleaching agents

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dc.contributor.authorAngelo Cintra, Luciano Tavares [UNESP]
dc.contributor.authorBenetti, Francine [UNESP]
dc.contributor.authorFerreira, Luciana Lousada [UNESP]
dc.contributor.authorRahal, Vanessa [UNESP]
dc.contributor.authorErvolino, Edilson [UNESP]
dc.contributor.authorJacinto, Rogerio de Castilho [UNESP]
dc.contributor.authorGomes Filho, Joao Eduardo [UNESP]
dc.contributor.authorFraga Briso, Andre Luiz [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2018-11-26T16:32:49Z
dc.date.available2018-11-26T16:32:49Z
dc.date.issued2016-01-01
dc.description.abstractDental materials, in general, are tested in different animal models prior to their clinical use in humans, except for bleaching agents. Objectives: To evaluate an experimental rat model for comparative studies of bleaching agents by investigating the influence of different concentrations and application times of H2O2 gel in the pulp tissue during in-office bleaching of rats' vital teeth. Material and methods: The right and left maxillary molars of 50 Wistar rats were bleached with 20% and 35% H(2)O(2)gels, respectively, for 5, 10, 15, 30, or 45 min (n=10 rats/group). Ten animals (control) were untreated. The rats were killed after 2 or 30 days, and the maxillae were examined by light microscopy. Inflammation was evaluated by histonnorphonnetric analysis with inflammatory cell counting in the corona| and radicular thirds of the pulp. The counting of fibroblasts was also performed. Scores were attributed to the odontoblastic layer and to vascular changes. The tertiary dentin area and the pulp chamber central area were histonnorphometrically measured. Data were compared by the analysis of variance and the Kruskal-Wallis test (p<0.05). Results: After 2 days, the amount of inflammatory cells increased in the occlusal third of the corona| pulp until the time of 15 min for both concentrations of bleaching gels. In 30 and 45 min groups of each concentration, the number of inflammatory cells decreased along with the appearance of necrotic areas. After 30 days, a reduction in the pulp chamber central area and an enlargement of tertiary dentin area were observed without the detection of inflammation areas. Conclusion: The rat model of extra coronal bleaching showed to be adequate for bleaching protocols studies, as it was possible to observe alterations in the pulp tissues and in the tooth structure caused by different concentrations and periods of application of bleaching agents.en
dc.description.affiliationUniv Estadual Paulista, UNESP, Fac Odontol Aracatuba, Dept Endodontia, Aracatuba, SP, Brazil
dc.description.affiliationUniv Estadual Paulista, UNESP, Fac Odontol Aracatuba, Dept Odontol Restauradora, Aracatuba, SP, Brazil
dc.description.affiliationUniv Estadual Paulista, UNESP, Fac Odontol Aracatuba, Dept Ciencias Basicas, Aracatuba, SP, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, UNESP, Fac Odontol Aracatuba, Dept Endodontia, Aracatuba, SP, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, UNESP, Fac Odontol Aracatuba, Dept Odontol Restauradora, Aracatuba, SP, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, UNESP, Fac Odontol Aracatuba, Dept Ciencias Basicas, Aracatuba, SP, Brazil
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIdFAPESP: 2011/13709-2
dc.description.sponsorshipIdFAPESP: 2013/25429-0
dc.format.extent95-104
dc.identifier.citationJournal Of Applied Oral Science. Bauru-sp: Univ Sao Paulo Fac Odontologia Bauru, v. 24, n. 1, p. 95-104, 2016.
dc.identifier.issn1678-7757
dc.identifier.lattes4408095517346846
dc.identifier.lattes3953630077047017
dc.identifier.orcid0000-0003-4859-0583
dc.identifier.orcid0000-0002-2362-8920
dc.identifier.urihttp://hdl.handle.net/11449/161458
dc.identifier.wosWOS:000375084600013
dc.language.isoeng
dc.publisherUniv Sao Paulo Fac Odontologia Bauru
dc.relation.ispartofJournal Of Applied Oral Science
dc.relation.ispartofsjr0,645
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectBleaching agents
dc.subjectAnimal models
dc.subjectHydrogen peroxide
dc.titleEvaluation of an experimental rat model for comparative studies of bleaching agentsen
dc.typeArtigo
dcterms.rightsHolderUniv Sao Paulo Fac Odontologia Bauru
unesp.author.lattes4408095517346846[5]
unesp.author.lattes5761956467234702[8]
unesp.author.lattes3953630077047017[6]
unesp.author.orcid0000-0001-5994-2287[7]
unesp.author.orcid0000-0003-4859-0583[5]
unesp.author.orcid0000-0002-6126-1760[8]
unesp.author.orcid0000-0002-2362-8920[6]
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Artigos - Odontologia Restauradora - FOA
Artigos - Ciências Básicas - FOA