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Crystallization and preliminary X-ray diffraction analysis of an acidic phospholipase A(2) complexed with p-bromophenacyl bromide and alpha-tocopherol inhibitors at 1.9-and 1.45-A resolution

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dc.contributor.authorTakeda, AAS
dc.contributor.authordos Santos, J. I.
dc.contributor.authorMarcussi, S.
dc.contributor.authorSilveira, L. B.
dc.contributor.authorSoares, A. M.
dc.contributor.authorFontes, MRM
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUNAERP
dc.date.accessioned2014-05-20T13:49:20Z
dc.date.available2014-05-20T13:49:20Z
dc.date.issued2004-06-01
dc.description.abstractAn acidic phospholipase A(2) (PLA(2)) isolated from Bothrops jararacussu snake venom was crystallized with two inhibitors: alpha-tocopherol (vitamin E) and p-bromophenacyl bromide (BPB). The crystals diffracted at 1.45- and 1.85-Angstrom resolution, respectively, for the complexes with alpha-tocopherol and p-bromophenacyl bromide. The crystals are not isomorphous with those of the native protein, suggesting the inhibitors binding was successful and changes in the quaternary structure may have occurred. (C) 2004 Elsevier B.V. All rights reserved.en
dc.description.affiliationUniv Estadual Paulista Julio Mesquita Filho, Inst Biociencias, Dept Fis & Biofis, BR-18618000 Botucatu, SP, Brazil
dc.description.affiliationUNAERP, Dept Biotecnol, Ribeirao Preto, SP, Brazil
dc.description.affiliationUnespUniv Estadual Paulista Julio Mesquita Filho, Inst Biociencias, Dept Fis & Biofis, BR-18618000 Botucatu, SP, Brazil
dc.format.extent281-284
dc.identifierhttp://dx.doi.org/10.1016/j.bbapap.2004.02.005
dc.identifier.citationBiochimica Et Biophysica Acta-proteins and Proteomics. Amsterdam: Elsevier B.V., v. 1699, n. 1-2, p. 281-284, 2004.
dc.identifier.doi10.1016/j.bbapap.2004.02.005
dc.identifier.issn1570-9639
dc.identifier.urihttp://hdl.handle.net/11449/17577
dc.identifier.wosWOS:000221807800030
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofBiochimica et Biophysica Acta: Proteins and Proteomics
dc.relation.ispartofjcr2.609
dc.relation.ispartofsjr1,170
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectcrystallizationpt
dc.subjectX-ray crystallographypt
dc.subjectacidic phospholipase A(2)pt
dc.subjectBothrops jararacussu venompt
dc.subjectalpha-tocopherolpt
dc.subjectp-bromophenacyl bromidept
dc.titleCrystallization and preliminary X-ray diffraction analysis of an acidic phospholipase A(2) complexed with p-bromophenacyl bromide and alpha-tocopherol inhibitors at 1.9-and 1.45-A resolutionen
dc.typeArtigo
dcterms.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dcterms.rightsHolderElsevier B.V.
dspace.entity.typePublication
unesp.author.orcid0000-0001-5000-6652[1]
unesp.author.orcid0000-0002-4634-6221[6]
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências, Botucatupt
unesp.departmentFísica e Biofísica - IBBpt

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