Immunomodulatory activity and control of Salmonella Enteritidis colonization in the intestinal tract of chickens by Lactobacillus based probiotic

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dc.contributor.authorPenha Filho, Rafael Antonio Casarin [UNESP]
dc.contributor.authorDíaz, Silvia Juliana Acelas [UNESP]
dc.contributor.authorFernando, Filipe Santos [UNESP]
dc.contributor.authorChang, Yung-Fu
dc.contributor.authorAndreatti Filho, Raphael Lucio [UNESP]
dc.contributor.authorBerchieri Junior, Angelo [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionCornell University
dc.date.accessioned2015-12-07T15:38:06Z
dc.date.available2015-12-07T15:38:06Z
dc.date.issued2015-09-15
dc.description.abstractLactobacillus-based probiotics (LBP) are used as competitive exclusion to control pathogenic enterobacterial infections and improve the weight gain in broiler chickens. This study assessed the inhibition of Salmonella Enteritidis (SE) infection in one-week-old broiler chicks, using an experimental LBP containing four Lactobacillus strains isolated from chickens (L. acidophilus, L. fermentum, L. reuteri, L. salivarius). The immunomodulatory effects of this treatment were evaluated, through the analysis of cytokines and influx of macrophages, γδ, CD4(+) and CD8(+) T cells in the gut. The intestinal colonization by SE was reduced by 1.8CFU/g (log10) in chicks treated with LBP (p<0.05). The levels of pro-inflammatory cytokines (IL-1β, LITAF) were significantly reduced in treated chicks (p<0.05), whilst untreated chicks showed elevated inflammatory stimulus and an increased population of CD8(+) T cells in the intestinal mucosa after challenge (p<0.05). Additionally, the LBP stimulated TLR2 expression in caecal tonsils. The adjuvant property of the Lactobacillus cell wall (LCW) was evaluated, demonstrating good capability to stimulate T helper 2 (Th2) cell proliferation. Pretreatment of chicks with LBP decreased the intestinal colonization by SE, minimizing the tissue lesions and inflammation after challenge and showed a potential use as adjuvant with injectable killed vaccines.en
dc.description.affiliationCollege of Veterinary Medicine – Cornell University, Ithaca, NY 14850, USA
dc.description.affiliationSchool of Agricultural and Veterinary Sciences – São Paulo State University (UNESP), Jaboticabal Campus, SP 14884-900, Brazil
dc.description.affiliationSchool of Veterinary Medicine and Animal – São Paulo State University (UNESP), Botucatu Campus, SP 18618-970, Brazil.
dc.description.affiliationUnespSchool of Agricultural and Veterinary Sciences – São Paulo State University (UNESP), Jaboticabal Campus, SP 14884-900, Brazil
dc.description.affiliationUnespSchool of Veterinary Medicine and Animal – São Paulo State University (UNESP), Botucatu Campus, SP 18618-970, Brazil.
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipFundação para o Desenvolvimento da UNESP (FUNDUNESP)
dc.description.sponsorshipIdFAPESP: 2012/24017-7
dc.description.sponsorshipIdFAPESP: 2013/26127-7
dc.format.extent64-69
dc.identifierhttp://dx.doi.org/10.1016/j.vetimm.2015.06.006
dc.identifier.citationVeterinary Immunology And Immunopathology, v. 167, n. 1-2, p. 64-69, 2015.
dc.identifier.doi10.1016/j.vetimm.2015.06.006
dc.identifier.issn1873-2534
dc.identifier.lattes3508096260678286
dc.identifier.pubmed26099807
dc.identifier.urihttp://hdl.handle.net/11449/131585
dc.language.isoeng
dc.publisherElsevier B. V.
dc.relation.ispartofVeterinary Immunology And Immunopathology
dc.rights.accessRightsAcesso restrito
dc.sourcePubMed
dc.subjectAdjuvanten
dc.subjectCytokineen
dc.subjectFowl paratyphoiden
dc.subjectMacrophageen
dc.subjectTlr2en
dc.subjectVaccineen
dc.titleImmunomodulatory activity and control of Salmonella Enteritidis colonization in the intestinal tract of chickens by Lactobacillus based probioticen
dc.typeArtigo
dcterms.rightsHolderElsevier B. V.
unesp.author.lattes3508096260678286[6]
unesp.author.orcid0000-0003-2522-6500[6]
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Ciências Agrárias e Veterinárias, Jaboticabalpt
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Medicina Veterinária e Zootecnia, Botucatupt
unesp.departmentPatologia Veterinária - FCAVpt

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Artigos - Clínica Veterinária - FMVZ
Jaboticabal - FCAV - Faculdade de Ciências Agrárias e Veterinárias