Nuclear transfer alters placental gene expression and associated histone modifications of the placental-specific imprinted gene pleckstrin homology-like domain, family A, member 2 (PHLDA2) in cattle

dc.contributor.authorArnold, Daniel R. [UNESP]
dc.contributor.authorGaspar, Roberta C. [UNESP]
dc.contributor.authorRocha, Carlos V. da [UNESP]
dc.contributor.authorSangalli, Juliano R.
dc.contributor.authorBem, Tiago H. C. de
dc.contributor.authorCorrea, Carolina A. P. [UNESP]
dc.contributor.authorPenteado, Joao C. T. [UNESP]
dc.contributor.authorMeirelles, Flavio V.
dc.contributor.authorLopes, Flavia L. [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionIn Vitro Brasil SA
dc.date.accessioned2018-11-26T15:43:40Z
dc.date.available2018-11-26T15:43:40Z
dc.date.issued2017-01-01
dc.description.abstractAbnormal placental development is frequent in nuclear transfer (NT) pregnancies and is likely to be associated with altered epigenetic reprogramming. In the present study, fetal and placental measurements were taken on Day 60 of gestation in cows with pregnancies produced by AI, IVF and NT. Placentas were collected and subjected to histological evaluation, the expression of genes important in trophoblast differentiation and expression of the placental imprinted gene pleckstrin homology-like domain, family A, member 2 (PHLDA2), as well as chromatin immunoprecipitation (ChIP) for histone marks within the promoter of PHLDA2. Fewer binucleated cells were observed in NT cotyledons, followed by IVF and AI cotyledons (P<0.05). Expression of heart and neural crest derivatives expressed 1 (HAND1), placental lactogen (PL), pregnancy-associated glycoprotein 9 (PAG-9) and PHLDA2 was elevated in NT cotyledons compared with AI cotyledons. Expression of PHLDA2 was higher in IVF than AI samples (P<0.05). ChIP revealed an increase in the permissive mark dimethylation of lysine 4 on histone H3 (H3K4me2), surprisingly associated with the silent allele of PHLDA2, and a decrease in the inhibitory mark H3K9me2 in NT samples. Thus, genes critical for placental development were altered in NT placentas, including an imprinted gene. Allele-specific changes in the permissive histone mark in the PHLDA2 promoter indicate misregulation of imprinting in clones. Abnormal trophoblast differentiation could have resulted in lower numbers of binucleated cells following NT. These results suggest that the altered expression of imprinted genes associated with NT are also caused by changes in histone modifications.en
dc.description.affiliationUniv Estadual Paulista, Sao Paulo State Univ, Fac Ciencias Agr & Vet, BR-14884900 Sao Paulo, Brazil
dc.description.affiliationUniv Sao Paulo, Fac Zootecnia & Engn Alimentos, BR-13635900 Sao Paulo, Brazil
dc.description.affiliationUniv Estadual Paulista, Sao Paulo State Univ, Fac Med Vet, BR-16050680 Sao Paulo, Brazil
dc.description.affiliationIn Vitro Brasil SA, BR-13800970 Sao Paulo, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, Sao Paulo State Univ, Fac Ciencias Agr & Vet, BR-14884900 Sao Paulo, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, Sao Paulo State Univ, Fac Med Vet, BR-16050680 Sao Paulo, Brazil
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipIdFAPESP: 2009/50381-5
dc.format.extent458-467
dc.identifierhttp://dx.doi.org/10.1071/RD15132
dc.identifier.citationReproduction Fertility And Development. Clayton: Csiro Publishing, v. 29, n. 3, p. 458-467, 2017.
dc.identifier.doi10.1071/RD15132
dc.identifier.issn1031-3613
dc.identifier.urihttp://hdl.handle.net/11449/159412
dc.identifier.wosWOS:000395555500003
dc.language.isoeng
dc.publisherCsiro Publishing
dc.relation.ispartofReproduction Fertility And Development
dc.relation.ispartofsjr0,681
dc.rights.accessRightsAcesso aberto
dc.sourceWeb of Science
dc.subjectassisted reproductive technologies
dc.subjectbovine
dc.subjectcotyledons
dc.subjectH3K4me2
dc.subjectH3K9me2
dc.subjectmRNA
dc.titleNuclear transfer alters placental gene expression and associated histone modifications of the placental-specific imprinted gene pleckstrin homology-like domain, family A, member 2 (PHLDA2) in cattleen
dc.typeArtigo
dcterms.rightsHolderCsiro Publishing

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