Chitosan and HPMCAS double-coating as protective systems for alginate microparticles loaded with Ctx(Ile21)-Ha antimicrobial peptide to prevent intestinal infections

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dc.contributor.authorRoque-Borda, Cesar Augusto [UNESP]
dc.contributor.authorSaraiva, Mauro de Mesquita Souza [UNESP]
dc.contributor.authorMacedo Junior, Wagner Dias [UNESP]
dc.contributor.authorMárquez Montesinos, José Carlos Estanislao
dc.contributor.authorMeneguin, Andréia Bagliotti [UNESP]
dc.contributor.authorToledo Borges, Anna Beatriz [UNESP]
dc.contributor.authorCrusca Junior, Edson [UNESP]
dc.contributor.authorGarrido, Saulo Santesso [UNESP]
dc.contributor.authorde Almeida, Adriana Maria [UNESP]
dc.contributor.authorMarchetto, Reinaldo [UNESP]
dc.contributor.authorChorilli, Marlus [UNESP]
dc.contributor.authorBerchieri Junior, Angelo [UNESP]
dc.contributor.authorTeixeira, Silvio Rainho [UNESP]
dc.contributor.authorPavan, Fernando Rogério [UNESP]
dc.contributor.authorVicente, Eduardo Festozo [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.contributor.institutionUniversidad de Talca
dc.date.accessioned2023-07-29T13:34:53Z
dc.date.available2023-07-29T13:34:53Z
dc.date.issued2023-02-01
dc.description.abstractThe incorrect use of conventional drugs for both prevention and control of intestinal infections has contributed to a significant spread of bacterial resistance. In this way, studies that promote their replacement are a priority. In the last decade, the use of antimicrobial peptides (AMP), especially Ctx(Ile21)-Ha AMP, has gained strength, demonstrating efficient antimicrobial activity (AA) against pathogens, including multidrug-resistant bacteria. However, gastrointestinal degradation does not allow its direct oral application. In this research, double-coating systems using alginate microparticles loaded with Ctx(Ile21)-Ha peptide were designed, and in vitro release assays simulating the gastrointestinal tract were evaluated. Also, the AA against Salmonella spp. and Escherichia coli was examined. The results showed the physicochemical stability of Ctx(Ile21)-Ha peptide in the system and its potent antimicrobial activity. In addition, the combination of HPMCAS and chitosan as a gastric protection system can be promising for peptide carriers or other low pH-sensitive molecules, adequately released in the intestine. In conclusion, the coated systems employed in this study can improve the formulation of new foods or biopharmaceutical products for specific application against intestinal pathogens in animal production or, possibly, in the near future, in human health.en
dc.description.affiliationSão Paulo State University (Unesp) School of Pharmaceutical Sciences, Araraquara
dc.description.affiliationSão Paulo State University (Unesp) School of Agricultural and Veterinarian Sciences, Jaboticabal
dc.description.affiliationSão Paulo State University (Unesp) School of Technology and Sciences, Presidente Prudente
dc.description.affiliationCentro de Bioinformática Simulación y Modelado (CBSM) Universidad de Talca
dc.description.affiliationSão Paulo State University (Unesp) Institute of Chemistry, Araraquara
dc.description.affiliationSão Paulo State University (Unesp) School of Sciences and Engineering, Tupã
dc.description.affiliationUnespSão Paulo State University (Unesp) School of Pharmaceutical Sciences, Araraquara
dc.description.affiliationUnespSão Paulo State University (Unesp) School of Agricultural and Veterinarian Sciences, Jaboticabal
dc.description.affiliationUnespSão Paulo State University (Unesp) School of Technology and Sciences, Presidente Prudente
dc.description.affiliationUnespSão Paulo State University (Unesp) Institute of Chemistry, Araraquara
dc.description.affiliationUnespSão Paulo State University (Unesp) School of Sciences and Engineering, Tupã
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIdFAPESP: 2016/00446–7
dc.description.sponsorshipIdFAPESP: 2018/25707–3
dc.description.sponsorshipIdFAPESP: 2020/16573–3
dc.identifierhttp://dx.doi.org/10.1016/j.biomaterials.2022.121978
dc.identifier.citationBiomaterials, v. 293.
dc.identifier.doi10.1016/j.biomaterials.2022.121978
dc.identifier.issn1878-5905
dc.identifier.issn0142-9612
dc.identifier.scopus2-s2.0-85145264489
dc.identifier.urihttp://hdl.handle.net/11449/248114
dc.language.isoeng
dc.relation.ispartofBiomaterials
dc.sourceScopus
dc.subjectAMP
dc.subjectChitosan
dc.subjectEnteric coating
dc.subjectHPMCAS
dc.subjectIn vitro release
dc.subjectMicroparticles
dc.titleChitosan and HPMCAS double-coating as protective systems for alginate microparticles loaded with Ctx(Ile21)-Ha antimicrobial peptide to prevent intestinal infectionsen
dc.typeArtigo
unesp.author.orcid0000-0002-9262-0383[1]
unesp.author.orcid0000-0002-9154-3574[15]
unesp.departmentAdministração - Tupãpt
unesp.departmentEstatística - FCTpt

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Presidente Prudente - FCT - Faculdade de Ciências e Tecnologia
Tupã - FCE - Faculdade de Ciências e Engenharia