Brazilian propolis antileishmanial and immunomodulatory effects

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dc.contributor.authorDa Silva, Suelen Santos
dc.contributor.authorThomé, Graciele Da Silva
dc.contributor.authorCataneo, Allan Henrique Depieri
dc.contributor.authorMiranda, Milena Menegazzo
dc.contributor.authorFelipe, Ionice
dc.contributor.authorAndrade, Célia Guadalupe Tardeli de Jesus
dc.contributor.authorWatanabe, Maria Angélica Ehara
dc.contributor.authorPiana, Gilce Maria [UNESP]
dc.contributor.authorSforcin, José Maurício [UNESP]
dc.contributor.authorPavanelli, Wander Rogério
dc.contributor.authorConchon-Costa, Ivete
dc.contributor.institutionUniversidade Estadual de Londrina (UEL)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-27T11:29:40Z
dc.date.available2014-05-27T11:29:40Z
dc.date.issued2013-06-12
dc.description.abstractThe antileishmanial and immunomodulatory effects of propolis collected in Botucatu, São Paulo State, Brazil, were evaluated in Leishmania (Viannia) braziliensis experimental infection. The antileishmanial effect of propolis on promastigote forms was verified by reducing growth and by promoting morphologic alterations observed by scanning electron microscopy. In in vitro immunomodulatory assays, macrophages were pretreated with propolis and then infected with L. (V.) braziliensis. In vivo, supernatants from liver cells and peritoneal exudate of BALB/c mice pretreated with propolis and infected with Leishmania (107/mL promastigotes) were collected, and TNF- α and IL-12 were measured by ELISA. Macrophages incubated with propolis showed a significant increase in interiorization and further killing of parasites. An increased TNF- α production was seen in mice pretreated with propolis, whereas IL-12 was downregulated during the infection. In conclusion, Brazilian propolis showed a direct action on the parasite and displayed immunomodulatory effects on murine macrophages, even though the parasite has been reported to affect the activation pathways of the cell. The observed effects could be associated with the presence of phenolic compounds (flavonoids, aromatic acids, and benzopyranes), di- and triterpenes, and essential oils found in our propolis sample. © 2013 Suelen Santos da Silva et al.en
dc.description.affiliationDepartment of Science Pathology Londrina State University (UEL), 86057-970 Londrina, PR
dc.description.affiliationDepartment of General Biology Laboratory of Electron Microscopy and Microanalysis Londrina State University (UEL), 86057-970 Londrina, PR
dc.description.affiliationDepartment of Microbiology and Immunology Biosciences Institute (UNESP), 18618-970 Botucatu, SP
dc.description.affiliationUnespDepartment of Microbiology and Immunology Biosciences Institute (UNESP), 18618-970 Botucatu, SP
dc.identifierhttp://dx.doi.org/10.1155/2013/673058
dc.identifier.citationEvidence-based Complementary and Alternative Medicine, v. 2013.
dc.identifier.doi10.1155/2013/673058
dc.identifier.file2-s2.0-84878695803.pdf
dc.identifier.issn1741-427X
dc.identifier.issn1741-4288
dc.identifier.scopus2-s2.0-84878695803
dc.identifier.urihttp://hdl.handle.net/11449/75640
dc.identifier.wosWOS:000319578600001
dc.language.isoeng
dc.relation.ispartofEvidence-based Complementary and Alternative Medicine
dc.relation.ispartofjcr2.064
dc.relation.ispartofsjr0,683
dc.rights.accessRightsAcesso aberto
dc.sourceScopus
dc.subjectantileishmanial agent
dc.subjectimmunomodulating agent
dc.subjectinterleukin 12
dc.subjectpropolis
dc.subjecttumor necrosis factor alpha
dc.subjectanimal experiment
dc.subjectanimal model
dc.subjectBrazil
dc.subjectcontrolled study
dc.subjectcytokine production
dc.subjectdown regulation
dc.subjectimmunomodulation
dc.subjectin vitro study
dc.subjectinfectious disease ELISA kit
dc.subjectLeishmania braziliensis
dc.subjectleishmaniasis
dc.subjectmacrophage
dc.subjectmale
dc.subjectmouse
dc.subjectnonhuman
dc.subjectpriority journal
dc.subjectscanning electron microscopy
dc.titleBrazilian propolis antileishmanial and immunomodulatory effectsen
dc.typeArtigo
dcterms.licensehttp://www.hindawi.com/journals/aaa/guidelines/
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências, Botucatupt

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Artigos - Microbiologia e Imunologia - IBB