Efficiency of Different Antimitotics in Cytological Preparations of Sugarcane

Melloni, Maria Natalia G. [UNESP]; Melloni, Maria Leticia G. [UNESP]; Neuber, Ana Caroline; Perecin, Dilermando [UNESP]; Landell, Marcos G. de Andrade; Pinto, Luciana R.

Efficiency of Different Antimitotics in Cytological Preparations of Sugarcane

Arquivos

WOS000371440000014.pdf (1.3 MB)

Data

2016-04-01

Autores

Melloni, Maria Natalia G.

Melloni, Maria Leticia G.

Neuber, Ana Caroline

Perecin, Dilermando

Landell, Marcos G. de Andrade

Pinto, Luciana R.

Editor

Springer

Tipo

Artigo

Direito de acesso

Acesso aberto

Resumo

Commercial sugarcane cultivars and wild species have a high ploidy level allied to a complex genome and usual chromosomal instability. There are few studies related to the standardization of cytogenetic techniques in sugarcane. This work tested the efficiency of three antimitotics (colchicine, 8-hydroxyquinoline, and trifuralin) at different concentrations on the quality of metaphases spreads from a commercial sugarcane cultivar (IAC911099-Saccharum spp.) and a wild sugarcane accession (Krakatau-S. spontaneum). Although all three antimitotics were efficient for obtaining metaphases, 8-hydroxyquinoline was the most efficient for both IAC911099 and Krakatau. The chromosome number of these two genotypes was inferred. The variation in chromosome number was 2n = 90-112 for IAC911099, with a statistical modal of 2n = 112 chromosomes while the variation in chromosome number was 2n = 90-129 chromosome with a statistical modal of 2n = 128 chromosomes for Krakatau (S. spontaneum).