Prevalence of Cryptosporidium infection in domestic cats from an urban area in Brazil

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dc.contributor.authorda Silveira Neto, Luiz
dc.contributor.authorWidmer, Giovanni
dc.contributor.authorde Lima, Valéria Marçal Felix [UNESP]
dc.contributor.authorMeireles, Marcelo Vasconcelos [UNESP]
dc.contributor.authorInácio, Sandra Valéria [UNESP]
dc.contributor.authorMarques, Marcel Gambin
dc.contributor.authorMarques, Ana Elisa Gregui Watanabe [UNESP]
dc.contributor.authorde Matos, Lucas Vinícius Shigaki
dc.contributor.authorda Cruz Panegossi, Mariele Fernanda
dc.contributor.authorBresciani, Katia Denise Saraiva [UNESP]
dc.contributor.institutionUFT
dc.contributor.institutionTufts University
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUNIFIO
dc.contributor.institutionIpanema Indústria de Produtos Veterinários
dc.contributor.institutionCentro Universitário Max Planck
dc.date.accessioned2021-06-25T10:34:11Z
dc.date.available2021-06-25T10:34:11Z
dc.date.issued2020-12-01
dc.description.abstractWe investigated the occurrence of Cryptosporidium oocysts shedding by domestic cats in an urban setting. The calculation of minimum sample size was based on an estimated prevalence of 10%, 5% absolute sampling error and a 5% significance level, resulting in 138 cats. A total of 612 owners of 2,290 cats had to be contacted for achieving the minimal sample size. In the end, only 55 owners accepted to participate in this investigation. Stool samples collected from 138 dogs were examined by microscopy using modified Kinyoun acid-fast staining, capture ELISA and nested-PCR followed by sequencing. Samples were considered positive when Cryptosporidium were detected by at least two diagnostic methods. Thirteen samples were positive (9.4%; 95% CI: 4.5 - 14.3). Cryptosporidium amplicons from seven out of the 13 samples were successfully sequenced and shared 99% genetic similarity to Cryptosporidium felis, GenBank access AF112575.1 was found. We concluded that Cryptosporidium infection is common in domestic cats from urban area and veterinary practitioners should guide cat owners to adopt preventive measures against the parasite to reduce the chance of infection in cats and householders.en
dc.description.affiliationEngenharia de Bioprocessos e Biotecnologia Universidade Federal do Tocantins UFT
dc.description.affiliationCummings School of Veterinary Medicine Tufts University
dc.description.affiliationUniversidade Estadual Paulista UNESP
dc.description.affiliationUNESP
dc.description.affiliationCentro Universitário das Faculdades Integradas de Ourinhos UNIFIO
dc.description.affiliationPós-Graduaçãoem Ciência Animal UNESP
dc.description.affiliationIpanema Indústria de Produtos Veterinários
dc.description.affiliationCurso de Medicina Veterinária Centro Universitário Max Planck
dc.description.affiliationUnespUniversidade Estadual Paulista UNESP
dc.description.affiliationUnespUNESP
dc.description.affiliationUnespPós-Graduaçãoem Ciência Animal UNESP
dc.format.extent2677-2685
dc.identifierhttp://dx.doi.org/10.5433/1679-0359.2020v41n6p2677
dc.identifier.citationSemina:Ciencias Agrarias, v. 41, n. 6, p. 2677-2685, 2020.
dc.identifier.doi10.5433/1679-0359.2020v41n6p2677
dc.identifier.issn1679-0359
dc.identifier.issn1676-546X
dc.identifier.scopus2-s2.0-85091415641
dc.identifier.urihttp://hdl.handle.net/11449/206554
dc.language.isoeng
dc.relation.ispartofSemina:Ciencias Agrarias
dc.sourceScopus
dc.subjectCryptosporidiosis
dc.subjectEpidemiology
dc.subjectFeline
dc.subjectPrevalence
dc.subjectPublic Health
dc.titlePrevalence of Cryptosporidium infection in domestic cats from an urban area in Brazilen
dc.titlePrevalência de infecção por Cryptosporidium em gatos domiciliados em uma área urbana no Brasilpt
dc.typeArtigo

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