The post-transcriptional gene silencing pathway in Eucalyptus
Data
2005-12-01
Orientador
Coorientador
Pós-graduação
Curso de graduação
Título da Revista
ISSN da Revista
Título de Volume
Editor
Tipo
Artigo
Direito de acesso
Acesso aberto
Resumo
Post-transcriptional gene silencing (PTGS) is a conserved surveillance mechanism that identifies and cleaves double-stranded RNA molecules and their cellular cognate transcripts. The RNA silencing response is actually used as a powerful technique (named RNA interference) for potent and specific inhibition of gene expression in several organisms. To identify gene products in Eucalyptus sharing similarities with enzymes involved in the PTGS pathway, we queried the expressed sequence tag database of the Brazilian Eucalyptus Genome Sequence Project Consortium (FORESTs) with the amino acid sequences of known PTGS-related proteins. Among twenty-six prospected genes, our search detected fifteen assembled sequences encoding products presenting high level of similarity (E value < 10 -40) to proteins involved in PTGS in plants and other organisms. We conclude that most of the genes known to be involved in the PTGS pathway are represented in the FORESTs database. Copyright by the Brazilian Society of Genetics.
Descrição
Palavras-chave
EST, Eucalyptus, Gene silencing, PTGS, RNA silencing, amino acid, enzyme, gene product, amino acid sequence, Brazil, controlled study, expressed sequence tag, genetic code, genetic database, genetic identification, genetic similarity, microbial genetics, nonhuman, nucleotide sequence, plant genetics, posttranscriptional gene silencing, protein assembly, sequence analysis
Idioma
Inglês
Como citar
Genetics and Molecular Biology, v. 28, n. 3 SUPPL., p. 496-500, 2005.