Effect of essential oils on Phomopsis sojae in vitro development

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Phomopsis sojae is considered the most directly involved fungus in the reduction of soybean seeds quality. Despite the proven effectiveness of chemicals products, the use of natural substances with fungicidal action, such as plant extracts and essential oils, has been investigated as an option of low ecological and environmental impact. Searching an alternative way of P. sojae control, this research aimed to verify the effect of essential oils on this fungus in vitro development. Essential oils (EOs) from eight plant species (neem, lemongrass, citronella, rosemary, citriodora eucalyptus, cloves, ginger and basil) were added to the PDA medium at four concentrations (0.25 %, 0.5%, 0.75% and 1.0%). In order to compare the EOs performance, two treatments were included, one composed of pure PDA medium (control) and another of medium containing thiophanate methyl + fluazinam (fungicide). The experiment was carried out on a completely randomized design, according to the 8x4 factorial scheme and two control treatments, with three replications per treatment. The experiment was performed in duplicate. Mycelial growth (cm) and sporulation (number of spores/ml) were evaluated. Lemongrass, cloves and basil EOs totally inhibited the fungal growth at all concentrations in both trials, not allowing sporulation. The other EOs didn’t show satisfactory performance as even inhibiting the fungal growth, they allowed high spores production, which is undesirable.




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Revista Brasileira de Plantas Medicinais, v. 20, n. 3, p. 288-296, 2018.

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