Rinsing with Statherin-Derived Peptide Alters the Proteome of the Acquired Enamel Pellicle

dc.contributor.authorTaira, Even Akemi
dc.contributor.authorFerrari, Carolina Ruis
dc.contributor.authorCarvalho, Gabriel
dc.contributor.authorVentura, Talita Mendes Oliveira
dc.contributor.authorMartini, Tatiana
dc.contributor.authorDionizio, Aline Salgado
dc.contributor.authorAraújo, Tamara Teodoro
dc.contributor.authorCrusca, Edson [UNESP]
dc.contributor.authorMarchetto, Reinaldo [UNESP]
dc.contributor.authorBuzalaf, Marília Afonso Rabelo
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.date.accessioned2022-04-29T08:45:39Z
dc.date.available2022-04-29T08:45:39Z
dc.date.issued2021-08-01
dc.description.abstractChanges in the proteomic profile of the acquired enamel pellicle (AEP) formed for 3 min or 2 h after rinsing with a peptide containing the 15 N-terminal residues of statherin, with serines 2 and 3 phosphorylated (StatpSpS), were evaluated. Nine volunteers participated in 2 consecutive days. Each day, after professional tooth cleaning, they rinsed for 1 min with 10 mL of phosphate buffer containing 1.88 × 10-5 M StatpSpS or phosphate buffer only (control). The acquired pellicle formed on enamel after 3 min or 2 h was collected with electrode filter papers soaked in 3% citric acid. After protein extraction, samples were analyzed by quantitative shotgun label-free proteomics. In the 3-min AEP, 19 and 131 proteins were uniquely identified in the StatpSpS and control groups, respectively. Proteins typically found in the AEP were only found in the latter. Only 2 proteins (neutrophil defensins) were increased upon treatment with StatpSpS, while 65 proteins (among which are several typical AEP proteins) were decreased. In the 2-h AEP, 50 and 108 proteins were uniquely found in StatpSpS and control groups, respectively. Hemoglobin subunits and isoforms of keratin were only found in the StatpSpS group, while cystatin-C, cathepsin D, and cathepsin G, isoforms of heat shock 70 and protocadherin were exclusively found in the control group. In addition, 23 proteins were increased upon treatment with StatpSpS, among which are histatin-1, serum albumin, and isoforms of neutrophil defensin and keratin, while 77 were decreased, most of them were typical AEP proteins. In both evaluated periods, rinsing with StatpSpS profoundly changed the proteomic profile of the AEP, which might impact the protective role of this integument against carious or erosive demineralization. This study provides important insights on the dynamics of the protein composition of the AEP along time, after rinsing with a solution containing StatpSpS.en
dc.description.affiliationDepartment of Biological Sciences Bauru School of Dentistry University of São Paulo
dc.description.affiliationChemistry Institute Paulista State University
dc.description.affiliationUnespChemistry Institute Paulista State University
dc.format.extent333-340
dc.identifierhttp://dx.doi.org/10.1159/000517959
dc.identifier.citationCaries Research, v. 55, n. 4, p. 333-340, 2021.
dc.identifier.doi10.1159/000517959
dc.identifier.issn1421-976X
dc.identifier.issn0008-6568
dc.identifier.scopus2-s2.0-85112386340
dc.identifier.urihttp://hdl.handle.net/11449/231491
dc.language.isoeng
dc.relation.ispartofCaries Research
dc.sourceScopus
dc.subjectAcquired pellicle
dc.subjectEnamel
dc.subjectErosive tooth wear
dc.subjectProteomics
dc.subjectStatherin
dc.titleRinsing with Statherin-Derived Peptide Alters the Proteome of the Acquired Enamel Pellicleen
dc.typeArtigo
unesp.departmentCiências Biológicas - FCpt

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