SIRT1-dependent effects of resveratrol and grape juice in an in vitro model of preeclampsia

dc.contributor.authorViana-Mattioli, Sarah [UNESP]
dc.contributor.authorCinegaglia, Naiara [UNESP]
dc.contributor.authorBertozzi-Matheus, Mariana [UNESP]
dc.contributor.authorBueno-Pereira, Thaina Omia [UNESP]
dc.contributor.authorCaldeira-Dias, Mayara [UNESP]
dc.contributor.authorCavalli, Ricardo Carvalho
dc.contributor.authorSandrim, Valeria Cristina [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.description.abstractPreeclampsia (PE) is a multifactorial hypertensive disorder of pregnancy that is partly responsible for both maternal and fetal morbidity and mortality levels worldwide. It has been recently discovered that sirtuin-1 (SIRT1) is reduced in the circulation and in an in vitro model of PE. Therefore, in this study, we investigated the effects of trans-resveratrol, a potent antioxidant and activator of SIRT1, on oxidative stress and nitric oxide (NO) production in an in vitro model of PE compared to gestational hypertensive (GH) and healthy pregnant (HP) women. Furthermore, we also evaluated the effects of an acute intake of grape juice on women with PE to assess whether it could mimic in vitro trans-resveratrol supplementation. (1) In the GH group, resveratrol decreased intracellular reactive oxygen species (ROS) and increased their antioxidant capacity, while inhibiting SIRT1 reestablished previous levels. (2) In PE, inhibition of SIRT1 increased antioxidant activity. (3) Intracellular NO and supernatant nitrite levels were increased by inhibiting SIRT1 in the PE group. (4) Grape juice intake increased intracellular NO levels versus before grape juice intake control; however, the inhibition of SIRT1 before grape juice intake initially increased NO, but decreased it 1 h after grape juice intake. In conclusion, activating SIRT1 by using resveratrol alone may not be beneficial to women with PE, and GH and PE seem to have different responsive mechanisms to this molecule. Furthermore, grape juice intake seems to have different effects compared to resveratrol supplementation alone in this in vitro model of PE, demonstrating the potential of the combination of other biologically active molecules from grape juice over the SIRT1-eNOS-NO in PE treatment.en
dc.description.affiliationDepartment of Biophysics and Pharmacology Institute of Biosciences of Botucatu Universidade Estadual Paulista (UNESP) Distrito Rubiao Junior Botucatu
dc.description.affiliationDepartment of Gynecology and Obstetrics Faculty of Medicine of Ribeirao Preto University of Sao Paulo Ribeirao Preto
dc.description.affiliationUnespDepartment of Biophysics and Pharmacology Institute of Biosciences of Botucatu Universidade Estadual Paulista (UNESP) Distrito Rubiao Junior Botucatu
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIdCNPq: 2014-5/305587
dc.description.sponsorshipIdFAPESP: 2015/20461-8
dc.description.sponsorshipIdFAPESP: 2019/06118-0
dc.description.sponsorshipIdFAPESP: 2019/07230-8
dc.description.sponsorshipIdCNPq: 301293/2018-0
dc.identifier.citationBiomedicine and Pharmacotherapy, v. 131.
dc.relation.ispartofBiomedicine and Pharmacotherapy
dc.subjectGrape juice
dc.subjectNitric oxide
dc.subjectOxidative stress
dc.titleSIRT1-dependent effects of resveratrol and grape juice in an in vitro model of preeclampsiaen