Anti-aquaporin-4 immunoglobulin G colorimetric detection by silver nanoparticles

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dc.contributor.authorHiga, Akemi M.
dc.contributor.authorMoraes, Ariana S.
dc.contributor.authorShimizu, Flávio M.
dc.contributor.authorBueno, Raquel G.
dc.contributor.authorPeroni, Luís A.
dc.contributor.authorStrixino, Francisco T.
dc.contributor.authorSousa, Nise A.C.
dc.contributor.authorDeffune, Elenice [UNESP]
dc.contributor.authorBovolato, Ana Lívia C. [UNESP]
dc.contributor.authorOliveira, Osvaldo N.
dc.contributor.authorBrum, Doralina G. [UNESP]
dc.contributor.authorLeite, Fabio L.
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Federal de São Carlos (UFSCar)
dc.contributor.institutionBrazilian Center for Research in Energy and Materials
dc.contributor.institutionRheabiotech Laboratory of Research and Development
dc.contributor.institutionUniversidade Federal do Amazonas
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.date.accessioned2022-05-01T13:57:34Z
dc.date.available2022-05-01T13:57:34Z
dc.date.issued2022-04-01
dc.description.abstractNeuromyelitis optica spectrum disorder (NMOSD) is an inflammatory and autoimmune disease whose biomarker is the anti-AQP4-IgG autoantibody that binds to aquaporin-4 (AQP4) protein. We introduced a nanosensor with a sensitivity of 84.6%, higher than the CBA's 76.5%. Using silver nanoparticles (AgNPs), we detected not only seropositive but also some false-negative patients previously classified with CBA. It consisted of AgNPs coated with one of a panel of 5 AQP4 epitopes. The ability in detecting the anti-AQP4-IgG in NMOSD patients depended on the epitope and synergy could be obtained by combining different epitopes. We demonstrated that NMOSD patients could easily be distinguished from healthy subjects and patients with multiple sclerosis. Using the most sensitive AQP461-70 peptide, we established a calibration curve to estimate the concentration of anti-AQP4-IgG in seropositive NMOSD patients. The ability to enhance the accuracy of the diagnosis may improve the prognosis of 10-27% of anti-AQP4-IgG seronegative patients worldwide.en
dc.description.affiliationUniversidade de São Paulo Instituto de Medicina Tropical, SP
dc.description.affiliationUniversidade Federal de São Carlos, SP
dc.description.affiliationBrazilian Nanotechnology National Laboratory Brazilian Center for Research in Energy and Materials, SP
dc.description.affiliationRheabiotech Laboratory of Research and Development, SP
dc.description.affiliationUniversidade Federal do Amazonas, AM
dc.description.affiliationUniversidade Estadual Paulista Júlio de Mesquita Filho Faculdade de Medicina, SP
dc.description.affiliationUniversidade de São Paulo Instituto de Física de São Carlos, SP
dc.description.affiliationUnespUniversidade Estadual Paulista Júlio de Mesquita Filho Faculdade de Medicina, SP
dc.identifierhttp://dx.doi.org/10.1016/j.nano.2022.102531
dc.identifier.citationNanomedicine: Nanotechnology, Biology, and Medicine, v. 41.
dc.identifier.doi10.1016/j.nano.2022.102531
dc.identifier.issn1549-9642
dc.identifier.issn1549-9634
dc.identifier.scopus2-s2.0-85125250875
dc.identifier.urihttp://hdl.handle.net/11449/234199
dc.language.isoeng
dc.relation.ispartofNanomedicine: Nanotechnology, Biology, and Medicine
dc.sourceScopus
dc.subjectAnti-aquaporin-4 immunoglobulin G
dc.subjectDiagnosis
dc.subjectEpitopes
dc.subjectNeuromyelitis optica spectrum disorders
dc.subjectSilver nanoparticles
dc.titleAnti-aquaporin-4 immunoglobulin G colorimetric detection by silver nanoparticlesen
dc.typeArtigo

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Artigos - Urologia - FMB