Interleukin-6, tumor necrosis factor-α, and CD5 immunolabeling of new experimental endodontic sealer and repair material

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dc.contributor.authorBenetti, Francine [UNESP]
dc.contributor.authorFerreira, Luciana Louzada [UNESP]
dc.contributor.authorDos Reis-Prado, Alexandre Henrique
dc.contributor.authorFaria, Flávio Duarte [UNESP]
dc.contributor.authorErvolino, Edilson [UNESP]
dc.contributor.authorBerbert, Fabio Luiz Camargo Vellela [UNESP]
dc.contributor.authorLeonardo, Renato de Toledo [UNESP]
dc.contributor.authorDias, João
dc.contributor.authorGomes-Filho, João Eduardo [UNESP]
dc.contributor.authorCintra, Luciano Tavares Angelo [UNESP]
dc.contributor.institutionUniversidade Federal de Minas Gerais (UFMG)
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.contributor.institutionUniversidade Estadual de Maringá (UEM)
dc.date.accessioned2023-03-01T20:52:33Z
dc.date.available2023-03-01T20:52:33Z
dc.date.issued2022-01-01
dc.description.abstractThe aim of this study was to evaluate the biocompatibility and immunoinflammatory response of the Sealepox and Sealepox-RP, based on interleukin (IL)-6, tumor necrosis factor (TNF)-α, and CD5 immunolabelling. The ProRoot MTA (PRMTA) was used for comparison. Polyethylene tubes (1.0-mm internal, 1.6-mm external diameter, and 10.0-mm length; ISO 10993) with or without (control) materials were randomly implanted in the dorsum of 35 rats (4 per rat). After 7, 15, 30, 60, and 90 days (n = 7), the tubes were removed for histological and immunohistochemical analysis. The Kruskal–Wallis and Dunn’s test for non-parametric data and, ANOVA and Tukey test for parametric data were used (P < 0.05). Hematoxylin and eosin staining revealed that the concentration of inflammatory cells decreased over time with no differences between groups in all periods (P > 0.05). Regarding IL-6 immunostaining, there was no difference at 7 days (P > 0.05); all groups decreased over time, being faster for the PRMTA group and also, with no differences between groups in the last period (P > 0.05). For TNF-α, at 7 days there was no difference between groups (P > 0.05); there was an increase at 15 days for PRMTA and, at 30 and 60 days, for PRMTA and Sealepox compared to the control (P < 0.05). At 90 days, Sealepox RP showed the lowest immunostaining being similar to the control (P > 0.05). Regarding CD5 cells, at 7 days, there was high immunostaining for PRMTA compared to the control (P < 0.05); and significant reduction over time with difference for all groups at 30 and 60 days. (P < 0.05); Sealepox was similar to the control in all periods (P > 0.05). Sealepox RP showed the highest immunostaining at 15 days, being different from the control and PRMTA (P < 0.05); in the other periods it was similar to the control (P > 0.05). It can be concluded that Sealepox and Sealepox-RP were biocompatible and demonstrated similar immunoinflammatory response regarding IL-6, TNF-α, and CD5 compared to PRMTA.en
dc.description.affiliationDepartment of Restorative Dentistry Faculdade de Odontologia Universidade Federal de Minas Gerais (UFMG), MG
dc.description.affiliationEndodontic Section Department of Preventive and Restorative Dentistry School of Dentistry São Paulo State University (Unesp), R: José Bonifácio, 1193. Vila Mendonça, São Paulo
dc.description.affiliationDepartment of Basic Science School of Dentistry São Paulo State University (Unesp), SP
dc.description.affiliationDepartment of Restorative Dentistry School of Dentistry São Paulo State University (Unesp), SP
dc.description.affiliationUniversity Institute Egas Moniz (IUEM), Monte de Caparica
dc.description.affiliationSchool of Dentistry Dental Assistance Center for Disabled Persons (CAOE) of the São Paulo State University (UNESP), SP
dc.description.affiliationUnespEndodontic Section Department of Preventive and Restorative Dentistry School of Dentistry São Paulo State University (Unesp), R: José Bonifácio, 1193. Vila Mendonça, São Paulo
dc.description.affiliationUnespDepartment of Basic Science School of Dentistry São Paulo State University (Unesp), SP
dc.description.affiliationUnespDepartment of Restorative Dentistry School of Dentistry São Paulo State University (Unesp), SP
dc.description.affiliationUnespSchool of Dentistry Dental Assistance Center for Disabled Persons (CAOE) of the São Paulo State University (UNESP), SP
dc.identifierhttp://dx.doi.org/10.1007/s10266-022-00723-7
dc.identifier.citationOdontology.
dc.identifier.doi10.1007/s10266-022-00723-7
dc.identifier.issn1618-1255
dc.identifier.issn1618-1247
dc.identifier.scopus2-s2.0-85132805284
dc.identifier.urihttp://hdl.handle.net/11449/241227
dc.language.isoeng
dc.relation.ispartofOdontology
dc.sourceScopus
dc.subjectBiocompatibility
dc.subjectExperimental sealers
dc.subjectImmune markers
dc.subjectInflammation
dc.subjectRepair material
dc.titleInterleukin-6, tumor necrosis factor-α, and CD5 immunolabeling of new experimental endodontic sealer and repair materialen
dc.typeArtigo
unesp.author.orcid0000-0002-5459-353X[1]
unesp.author.orcid0000-0002-5866-7137[3]
unesp.author.orcid0000-0003-4269-7902[4]
unesp.author.orcid0000-0003-4859-0583[5]
unesp.author.orcid0000-0003-3292-3136[6]
unesp.author.orcid0000-0002-0672-9981[7]
unesp.author.orcid0000-0001-5994-2287[9]
unesp.author.orcid0000-0003-2348-7846[10]

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Araçatuba - CAOE - Centro de Assistência Odontológica a Excepcionais