Consequences of endogenous and exogenous WNT signaling for development of the preimplantation bovine embryo

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dc.contributor.authorTribulo, Paula
dc.contributor.authorda Silva Leão, Beatriz Caetano [UNESP]
dc.contributor.authorLehloenya, Khoboso C.
dc.contributor.authorMingoti, Gisele Zoccal [UNESP]
dc.contributor.authorHansen, Peter J.
dc.contributor.institutionUniversity of Florida
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversity of Pretoria
dc.date.accessioned2018-12-11T17:33:58Z
dc.date.available2018-12-11T17:33:58Z
dc.date.issued2017-01-01
dc.description.abstractThe specific role of WNT signaling during preimplantation development remains unclear. Here, we evaluated consequences of activation and inhibition of ß-catenin (CTNNB1)-dependent and -independent WNT signaling in the bovine preimplantation embryo. Activation of CTNNB1- mediated WNT signaling by the agonist 2-amino-4-(3,4-(methylenedioxy)benzylamino)-6-(3- methoxyphenyl)pyrimidine (AMBMP) and a glycogen synthase kinase 3 inhibitor reduced development to the blastocyst stage. Moreover, the antagonist of WNT signaling, dickkopf-related protein 1 (DKK1), alleviated the negative effect of AMBMP on development via reduction of CTNNB1. Based on labeling for phospho c-Jun N-terminal kinase, there was no evidence that DKK1 activated the planar cell polarity (PCP) pathway. Inhibition of secretion of endogenous WNTs did not affect development but increased number of cells in the inner cell mass (ICM). In contrast, DKK1 did not affect number of ICM or trophectoderm (TE) cells, suggesting that embryo-derived WNTs regulate ICM proliferation through a mechanism independent of CTNNB1. In addition, DKK1 did not affect the number of cells positive for the transcription factor yes-associated protein 1 (YAP1) involved in TE formation. In fact, DKK1 decreased YAP1. In contrast, exposure of embryos to WNT family member 7A (WNT7A) improved blastocyst development, inhibited the PCP pathway, and did not affect amounts of CTNNB1. Results indicate that embryo-derived WNTs are dispensable for blastocyst formation but participate in regulation of ICM proliferation, likely through a mechanism independent of CTNNB1. The response to AMBMP and WNT7A leads to the hypothesis that maternally derived WNTs can play a positive or negative role in regulation of preimplantation development.en
dc.description.affiliationDepartment of Animal Sciences D.H. Barron Reproductive and Perinatal Biology Research Program Genetics Institute University of Florida
dc.description.affiliationSchool of Veterinary Medicine Laboratory of Reproductive Physiology UNESP-Universidade Estadual Paulista
dc.description.affiliationSchool of Agrarian and Veterinarian Sciences Department of Animal Reproduction UNESP-Universidade Estadual Paulista
dc.description.affiliationDepartment of Animal and Wildlife Sciences University of Pretoria
dc.description.affiliationUnespSchool of Veterinary Medicine Laboratory of Reproductive Physiology UNESP-Universidade Estadual Paulista
dc.description.affiliationUnespSchool of Agrarian and Veterinarian Sciences Department of Animal Reproduction UNESP-Universidade Estadual Paulista
dc.description.sponsorshipNational Institutes of Health
dc.description.sponsorshipIdNational Institutes of Health: R03 HD080855
dc.format.extent1129-1141
dc.identifierhttp://dx.doi.org/10.1093/biolre/iox048
dc.identifier.citationBiology of Reproduction, v. 96, n. 6, p. 1129-1141, 2017.
dc.identifier.doi10.1093/biolre/iox048
dc.identifier.file2-s2.0-85028955871.pdf
dc.identifier.issn1529-7268
dc.identifier.issn0006-3363
dc.identifier.scopus2-s2.0-85028955871
dc.identifier.urihttp://hdl.handle.net/11449/179154
dc.language.isoeng
dc.relation.ispartofBiology of Reproduction
dc.relation.ispartofsjr1,446
dc.relation.ispartofsjr1,446
dc.rights.accessRightsAcesso aberto
dc.sourceScopus
dc.subjectDKK1
dc.subjectEmbryo development
dc.subjectPreimplantation development
dc.subjectWNT
dc.subjectWNT7A
dc.titleConsequences of endogenous and exogenous WNT signaling for development of the preimplantation bovine embryoen
dc.typeArtigo

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