Antitumor effect of Bothrops jararaca venom

dc.contributor.authorda Silva, R. J.
dc.contributor.authorda Silva, M. G.
dc.contributor.authorVilela, L. C.
dc.contributor.authorFecchio, D.
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-20T15:28:07Z
dc.date.available2014-05-20T15:28:07Z
dc.date.issued2002-04-01
dc.description.abstractMANY experimental studies have been carried out using snake venoms for the treatment of animal tumors, with controversial results. While some authors have reported an antitumor effect of treatment with specific snake venom fractions, others have reported no effects after this treatment. The aim of this study was to evaluate the effect of Bothrops jararaca venom (BjV) on Ehrlich ascites tumor (EAT) cells in vivo and in vitro. In the in vivo study, Swiss mice were inoculated with EAT cells by the intraperitoneal (i.p.) route and treated with BjV venom (0.4 mg/kg, i.p.), on the 1st, 4th, 7th, 10th, and 13th days. Mice were evaluated for total and differential cells number on the 2nd, 5th, 8th, 11th and 14th days. The survival time was also evaluated after 60 days of tumor growth. In the in vitro study, EAT and normal peritoneal cells were cultivated in the presence of different BjV concentrations (2.5, 5.0, 10.0, 20.0, 40.0, and 80 mug) and viability was verified after 3, 6, 12 and 24 h of cultivation. Results were analyzed statistically by the Kruskal-Wallis and Tukey tests at the 5% level of significance. It was observed that in vivo treatment with BjV induced tumor growth inhibition, increased animal survival time, decreased mortality, increased the influx of polymorphonuclear leukocytes on the early stages of tumor growth, and did not affect the mononuclear cells number. In vitro treatment with BjV produced a dose-dependent toxic effect on EAT and peritoneal cells, with higher effects against peritoneal cells. Taken together, our results demonstrate that BjV has an important antitumor effect. This is the first report showing this in vivo effect for this venom.en
dc.description.affiliationUNESP, Fac Med, Dept Patol, BR-18618000 São Paulo, Brazil
dc.description.affiliationUNESP, Inst Biociencias, Dept Parasitol, BR-18618000 São Paulo, Brazil
dc.description.affiliationUnespUNESP, Fac Med, Dept Patol, BR-18618000 São Paulo, Brazil
dc.description.affiliationUnespUNESP, Inst Biociencias, Dept Parasitol, BR-18618000 São Paulo, Brazil
dc.format.extent99-104
dc.identifierhttp://dx.doi.org/10.1080/09629350220131953
dc.identifier.citationMediators of Inflammation. Basingstoke: Carfax Publishing, v. 11, n. 2, p. 99-104, 2002.
dc.identifier.doi10.1080/09629350220131953
dc.identifier.fileWOS000175495000006.pdf
dc.identifier.issn0962-9351
dc.identifier.urihttp://hdl.handle.net/11449/38004
dc.identifier.wosWOS:000175495000006
dc.language.isoeng
dc.publisherCarfax Publishing
dc.relation.ispartofMediators of Inflammation
dc.relation.ispartofjcr3.549
dc.relation.ispartofsjr1,370
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectantitumor effectpt
dc.subjectEhrlich ascites tumorpt
dc.subjectBothrops jararacapt
dc.subjectvenompt
dc.titleAntitumor effect of Bothrops jararaca venomen
dc.typeArtigo
dcterms.licensehttp://www.hindawi.com/oa/
dcterms.rightsHolderCarfax Publishing
unesp.author.orcid0000-0002-3426-6873[2]
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências, Botucatupt
unesp.departmentParasitologia - IBBpt

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