Generation and Characterization of a Soybean Line with a Vernonia galamensis Diacylglycerol Acyltransferase-1 Gene and a myo-Inositol 1-Phosphate Synthase Knockout Mutation

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dc.contributor.authorAL-Amery, Maythem
dc.contributor.authorFowler, Ashley
dc.contributor.authorUnrine, Jason M.
dc.contributor.authorArmstrong, Paul
dc.contributor.authorMaghirang, Elizabeth
dc.contributor.authorSu, Kai
dc.contributor.authorde Melo, Julia [UNESP]
dc.contributor.authorYuan, Feng-Jie
dc.contributor.authorShu, Qing-Yao
dc.contributor.authorHildebrand, David
dc.contributor.institutionUniversity of Baghdad
dc.contributor.institutionUniversity of Kentucky
dc.contributor.institutionSPIERU-CGAHR
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionZhejiang University
dc.date.accessioned2020-12-12T01:27:33Z
dc.date.available2020-12-12T01:27:33Z
dc.date.issued2020-01-01
dc.description.abstractSoybean (Glycine max) meal is an important protein source. Soybean meal with lower phytate and oligosaccharides improves meal quality. A single recessive mutation in soybean myo-inositol 1-phosphate synthase (Gm-lpa-TW75-1) confers a seed phenotype with low phytate and increased inorganic phosphate. The mutant was crossed with high oil lines expressing a diacylglycerol acyltransferase1 (DGAT) gene from Vernonia galamensis (VgD). Gm-lpa-TW75-1 X VgD, designated GV, has 21%, and 22% oil and 41% and 43% protein from field and greenhouse seed production, respectively. No significant differences were found in mineral concentrations except for Fe which was 229 μg/g dry mass for GV followed by 174.3 for VgD and 162 for Gm-lpa-TW75-1. Phosphate (Pi) is higher in Gm-lpa-TW75-1 as expected at 5 mg/g, followed by GV at 1.6 mg/g whereas Jack, VgD, and Taiwan75 have about 0.3 mg/g. The Gm-lpa-TW75-1 line has the lowest phytate concentration at 1.4 mg/g followed by GV with 1.8 mg/g compared to Taiwan75, VgD, and Jack with 2.5 mg/g. This work describes a high oil and protein soybean line, GV, with increased Pi and lower phytate which will increase the nutritional value for human and animal feed.en
dc.description.affiliationDepartment of Biology College of Science for Women University of Baghdad, Karrada, Al-Jadriya, 10071
dc.description.affiliationDepartment of Plant and Soil Sciences University of Kentucky, Plant Science Building, 1405 Veterans Drive
dc.description.affiliationDepartment of Animal and Food Sciences University of Kentucky
dc.description.affiliationUSDA-ARS SPIERU-CGAHR
dc.description.affiliationUNESP Campus Jaboticabal State of Sao Paulo 295 9 de julho street Conchal
dc.description.affiliationZhejiang Provincial Key Laboratory for Germplasm Resources Institute of Crop Science Zhejiang University
dc.description.affiliationUnespUNESP Campus Jaboticabal State of Sao Paulo 295 9 de julho street Conchal
dc.identifierhttp://dx.doi.org/10.1002/lipd.12253
dc.identifier.citationLipids.
dc.identifier.doi10.1002/lipd.12253
dc.identifier.issn1558-9307
dc.identifier.issn0024-4201
dc.identifier.scopus2-s2.0-85086442106
dc.identifier.urihttp://hdl.handle.net/11449/198985
dc.language.isoeng
dc.relation.ispartofLipids
dc.sourceScopus
dc.subjectGlycine max
dc.subjectHigh oil
dc.subjectHigh phosphate
dc.subjectHigh protein
dc.subjectLow phytate
dc.titleGeneration and Characterization of a Soybean Line with a Vernonia galamensis Diacylglycerol Acyltransferase-1 Gene and a myo-Inositol 1-Phosphate Synthase Knockout Mutationen
dc.typeArtigo

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