Mechanism for the uncoupling of oxidative phosphorylation by juliprosopine on rat brain mitochondria

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dc.contributor.authorMaioli, Marcos A. [UNESP]
dc.contributor.authorLemos, Danilo E. C. V.
dc.contributor.authorGuelfi, Marieli [UNESP]
dc.contributor.authorMedeiros, Hyllana C. D. [UNESP]
dc.contributor.authorRiet-Correa, Franklin
dc.contributor.authorMedeiros, Rosane M. T.
dc.contributor.authorBarbosa-Filho, Jose M.
dc.contributor.authorMingatto, Fábio Erminio [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade Federal de Campina Grande (UFCG)
dc.contributor.institutionUniversidade Federal da Paraíba (UFPB)
dc.date.accessioned2014-05-20T15:30:19Z
dc.date.available2014-05-20T15:30:19Z
dc.date.issued2012-12-15
dc.description.abstractProsopis juliflora, popularly known as Algaroba, is a major problem because the lack of food during the driest times of the year and its high palatability and nutritional value make its fruits (pods) much appreciated by cattle, goats, sheep and other animals. However, the consumption of this plant for long periods can cause a disease called cara-torta (pie face), which is characterized by cranial nerve dysfunction, mainly due to the degeneration and disappearance of neurons in the trigeminal motor nucleus. Algaroba contains piperidine alkaloids that have been suggested as being responsible for its toxicity; one of these alkaloids is juliprosopine. This study was conducted to evaluate the mechanisms of action of juliprosopine in isolated rat brain mitochondria to evaluate the potential mechanisms that lead to neurotoxicity in animals intoxicated by algaroba. Juliprosopine stimulated state-4 respiration at concentrations of 10-25 mu M, affected the membrane potential at all concentrations studied (5-25 mu M) and affected ATP production only at higher concentrations (15 and 25 mu M). Juliprosopine cannot be classified as a member of the protonophoric class of uncouplers, such as 2,4-dinitrophenol or CCCP (m-chlorophenylhydrazone). due to its inability to promote mitochondrial swelling in the hyposmotic medium of potassium acetate. in addition, carboxyatractyloside, Mg2+, cyclosporine A and dithiothreitol did not protect the uncoupling induced by juliprosopine. Because juliprosopine increased the fluorescence responses of mitochondria labeled with 1-aniline-8-naphthalene sulfonate (ANS) and DPH (1,6-diphenyl-1,3,5-hexatriene), we suggested that its uncoupling action must be attributed to a modification of the arrangement of the inner mitochondrial membrane. (C) 2012 Elsevier Ltd. All rights reserved.en
dc.description.affiliationUniv Estadual Paulista, UNESP, Lab Bioquim Metabol & Toxicol LaBMeT, BR-17900000 Dracena, SP, Brazil
dc.description.affiliationUniv Fed Campina Grande, UFCG, CSTR, Hosp Vet, BR-58700000 Patos de Minas, PB, Brazil
dc.description.affiliationUniversidade Federal da Paraíba (UFPB), UFPB, Lab Tecnol Farmaceut, BR-58051900 Joao Pessoa, PB, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, UNESP, Lab Bioquim Metabol & Toxicol LaBMeT, BR-17900000 Dracena, SP, Brazil
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipNational Institute for the Control of Plant Poisoning
dc.description.sponsorshipIdNational Institute for the Control of Plant Poisoning: 573534/2008-0
dc.format.extent1355-1362
dc.identifierhttp://dx.doi.org/10.1016/j.toxicon.2012.09.012
dc.identifier.citationToxicon. Oxford: Pergamon-Elsevier B.V. Ltd, v. 60, n. 8, p. 1355-1362, 2012.
dc.identifier.doi10.1016/j.toxicon.2012.09.012
dc.identifier.issn0041-0101
dc.identifier.urihttp://hdl.handle.net/11449/39735
dc.identifier.wosWOS:000312509200004
dc.language.isoeng
dc.publisherPergamon-Elsevier B.V. Ltd
dc.relation.ispartofToxicon
dc.relation.ispartofjcr2.352
dc.relation.ispartofsjr0,692
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectProsopis julifloraen
dc.subjectJuliprosopineen
dc.subjectNeurotoxicityen
dc.subjectMitochondriaen
dc.subjectBioenergeticsen
dc.titleMechanism for the uncoupling of oxidative phosphorylation by juliprosopine on rat brain mitochondriaen
dc.typeArtigo
dcterms.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dcterms.rightsHolderPergamon-Elsevier B.V. Ltd
unesp.author.orcid0000-0003-3488-1814[8]
unesp.author.orcid0000-0002-9567-4096[7]
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Ciências Agrárias e Tecnológicas, Dracenapt

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