Protective effect of nickel chloride on superoxide damage: enhancement of CuZn superoxide dismutase affinity to the oxygen free radical.

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dc.contributor.authorNovelli, E. L.
dc.contributor.authorRodrigues, N. L.
dc.contributor.authorRibas, B. O.
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-27T11:17:31Z
dc.date.available2014-05-27T11:17:31Z
dc.date.issued1993-01-01
dc.description.abstractThe effect of nickel from soluble NiCl2 on Cu-Zn superoxide dismutase (SOD) activity, as well as on rate of nitro blue tetrazolium reduction, was studied in vitro since lipid peroxidation has been implicated in cell damage by nickel insoluble compounds, whose toxicity and carcinogenicity are well established. The physical and chemical nature of nickel compounds is one of the key determinations of its toxicity. Soluble nickel freely enter cells, but is just as readily excreted reducing the opportunity for production of lipid damage. Nickel from NiCl2 strongly activated SOD activity. In vitro addition of nickel chloride to a crude lung preparation altered the KM for SOD without changing the Vmax. Nickel chloride produced increased enzyme affinity to the substrate, because decreased (O2-) concentration that yields half-maximal velocity. The combination of nickel and SOD may contribute to stabilization of the particular conformation of SOD responsible for maximal catalytically activity.en
dc.format.extent28-32
dc.identifier.citationBoletin de estudios medicos y biologicos, v. 41, n. 1-4, p. 28-32, 1993.
dc.identifier.issn0067-9666
dc.identifier.scopus2-s2.0-0027341630
dc.identifier.urihttp://hdl.handle.net/11449/64350
dc.language.isoeng
dc.relation.ispartofBoletin de estudios medicos y biologicos
dc.rights.accessRightsAcesso restrito
dc.sourceScopus
dc.subjectantioxidant
dc.subjectnickel
dc.subjectnickel chloride
dc.subjectnitroblue tetrazolium
dc.subjectreactive oxygen metabolite
dc.subjectsuperoxide dismutase
dc.subjectair pollutant
dc.subjectanimal
dc.subjectchemistry
dc.subjectdrug effect
dc.subjectenzyme activation
dc.subjectenzymology
dc.subjectlung
dc.subjectmale
dc.subjectmetabolism
dc.subjectoxidation reduction reaction
dc.subjectprotein binding
dc.subjectrat
dc.subjectrat strain
dc.subjectAir Pollutants, Environmental
dc.subjectAnimal
dc.subjectAntioxidants
dc.subjectEnzyme Activation
dc.subjectLung
dc.subjectMale
dc.subjectNickel
dc.subjectNitroblue Tetrazolium
dc.subjectOxidation-Reduction
dc.subjectProtein Binding
dc.subjectRats
dc.subjectRats, Wistar
dc.subjectReactive Oxygen Species
dc.subjectSuperoxide Dismutase
dc.titleProtective effect of nickel chloride on superoxide damage: enhancement of CuZn superoxide dismutase affinity to the oxygen free radical.en
dc.typeArtigo

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