Anaplasma marginale in goats from a multispecies grazing system in northeastern Brazil

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dc.contributor.authorBarbosa, Iago C.
dc.contributor.authorAndré, Marcos R. [UNESP]
dc.contributor.authorAmaral, Renan Bressianini do [UNESP]
dc.contributor.authorValente, Jessica D.M.
dc.contributor.authorVasconcelos, Priscylla C.
dc.contributor.authorOliveira, Celso J.B.
dc.contributor.authorJusi, Marcia Mariza Gomes
dc.contributor.authorMachado, Rosangela Z. [UNESP]
dc.contributor.authorVieira, Thállitha S.W.J.
dc.contributor.authorUeti, Massaro W.
dc.contributor.authorVieira, Rafael F.C.
dc.contributor.institutionUniversidade Estadual de Londrina (UEL)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade Federal do Paraná (UFPR)
dc.contributor.institutionUniversidade Federal da Paraíba (UFPB)
dc.contributor.institutionImunodot Diagnósticos
dc.contributor.institutionU.S. Department of Agriculture
dc.contributor.institutionWashington State University
dc.contributor.institutionThe Ohio State University
dc.date.accessioned2021-06-25T10:36:53Z
dc.date.available2021-06-25T10:36:53Z
dc.date.issued2021-01-01
dc.description.abstractAnaplasma marginale, a tick-borne α-proteobacterium that causes significant economic losses for the cattle industry worldwide, has been increasingly detected in other animal species. This agent has been previously detected in buffaloes and goats co-grazed with cattle in Brazil. This study aimed to investigate the occurrence of A. marginale in a multispecies (goats, sheep and cattle) grazing farm in the State of Paraíba, northeastern Brazil. A total of 119 goats, 71 sheep, and five cattle were evaluated. An epidemiological questionnaire was applied to the farm owner addressing age, gender, and presence of ticks. Serum samples from goat, sheep and cattle were tested for anti-Anaplasma marginale antibodies by a commercial MSP5-based on indirect enzyme-linked immunosorbent assay (iELISA). EDTA-blood samples were screened for A. marginale- and A. ovis-infection by PCR using primers targeting Anaplasma spp. msp4 gene. Sequencing of the repeat region of the msp1α gene was used for genotyping A. marginale strains found in the present study. A total of 47/119 (39.5 %, 95 % CI: 31.1–48.4 %) goats and 2/71 (3%, 95 % CI: 0.7–9.7 %) sheep were seroreactive for A. marginale rMSP5 by the commercial iELISA. All cattle were seronegative for A. marginale. Anaplasma spp. msp4 PCR results revealed that two out of 119 (1.7 %; 95 % CI: 0.4–5.9 %) goats tested positive and all sheep and cattle samples were negative. It was not possible to sequence one sample. Therefore, the other sequencing sample found tandem repeats of A. marginale msp1α gene demonstrating that goat was infected with the genotype F/91. Rhipicephalus microplus ticks were found parasitizing goats but not on sheep or cattle. Considering that in Brazil A. marginale genotype F/91 and the MSP1a tandem repeat F has only been detected in goats so far, we hypothesized that this genotype may be related to goats.en
dc.description.affiliationPrograma de Pós-Graduação em Ciência Animal Universidade Estadual de Londrina
dc.description.affiliationLaboratório de Imunoparasitologia Departamento de Patologia Veterinária Faculdade de Ciências Agrárias e Veterinárias Universidade Estadual Paulista (FCAV/UNESP)
dc.description.affiliationVector-Borne Diseases Laboratory Departament of Veterinary Medicine Universidade Federal do Paraná
dc.description.affiliationDepartment of Animal Science Center for Agricultural Sciences Universidade Federal da Paraíba
dc.description.affiliationImunodot Diagnósticos
dc.description.affiliationAnimal Diseases Research Unit Agricultural Research Service U.S. Department of Agriculture
dc.description.affiliationDepartment of Veterinary Microbiology and Pathology Washington State University
dc.description.affiliationGlobal One Health Initiative (GOHi) The Ohio State University
dc.description.affiliationUnespLaboratório de Imunoparasitologia Departamento de Patologia Veterinária Faculdade de Ciências Agrárias e Veterinárias Universidade Estadual Paulista (FCAV/UNESP)
dc.identifierhttp://dx.doi.org/10.1016/j.ttbdis.2020.101592
dc.identifier.citationTicks and Tick-borne Diseases, v. 12, n. 1, 2021.
dc.identifier.doi10.1016/j.ttbdis.2020.101592
dc.identifier.issn1877-9603
dc.identifier.issn1877-959X
dc.identifier.scopus2-s2.0-85093066167
dc.identifier.urihttp://hdl.handle.net/11449/206707
dc.language.isoeng
dc.relation.ispartofTicks and Tick-borne Diseases
dc.sourceScopus
dc.subjectAnaplasmosis
dc.subjectCattle
dc.subjectGoat
dc.subjectmsp1α and msp4
dc.subjectRhipicephalus microplus
dc.titleAnaplasma marginale in goats from a multispecies grazing system in northeastern Brazilen
dc.typeArtigo
unesp.departmentPatologia Veterinária - FCAVpt

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Jaboticabal - FCAV - Faculdade de Ciências Agrárias e Veterinárias