Experimental Bothrops atrox Envenomation: Blood Plasma Proteome Effects after Local Tissue Damage and Perspectives on Thromboinflammation

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dc.contributor.authorCavalcante, Joeliton S. [UNESP]
dc.contributor.authorBrito, Ingrid Mayara da Cunha [UNESP]
dc.contributor.authorDe Oliveira, Laudicéia Alves [UNESP]
dc.contributor.authorDe Barros, Luciana Curtolo [UNESP]
dc.contributor.authorAlmeida, Cayo
dc.contributor.authorRossini, Bruno Cesar [UNESP]
dc.contributor.authorSousa, Duaran Lopes
dc.contributor.authorAlves, Renata Sousa
dc.contributor.authorJorge, Roberta Jeane Bezerra
dc.contributor.authorSantos, Lucilene Delazari dos [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.contributor.institutionFederal University of ABC
dc.contributor.institutionFederal University of Ceará
dc.contributor.institutionFederal University of Ceará (UFC)
dc.date.accessioned2023-07-29T13:21:47Z
dc.date.available2023-07-29T13:21:47Z
dc.date.issued2022-09-01
dc.description.abstractThe clinical manifestations of Bothrops atrox envenoming involve local and systemic changes, among which edema requires substantial attention due to its ability to progress to compartmental syndromes and sometimes cause tissue loss and amputations. However, the impact of edema on the poisoned body’s system has not been explored. Thus, the present study aimed to explore the systemic pathological and inflammatory events that are altered by intraplantar injection of B. atrox venom in a mouse model through hematologic, lipidic, and shotgun proteomics analysis. Plasma samples collected showed a greater abundance of proteins related to complement, coagulation, lipid system, platelet and neutrophil degranulation, and pathways related to cell death and ischemic tolerance. Interestingly, some proteins, in particular, Prdx2 (peroxiredoxin 2), Hba (hemoglobin subunit alpha), and F9 (Factor IX), increased according to the amount of venom injected. Our findings support that B. atrox venom activates multiple blood systems that are involved in thromboinflammation, an observation that may have implications for the pathophysiological progression of envenomations. Furthermore, we report for the first time a potential role of Prdx2, Hba, and F9 as potential markers of the severity of edema/inflammation in mice caused by B. atrox.en
dc.description.affiliationGraduate Program in Tropical Diseases Botucatu Medical School (FMB) São Paulo State University (UNESP)
dc.description.affiliationCenter for the Study of Venoms and Venomous Animals (CEVAP) São Paulo State University (UNESP)
dc.description.affiliationCenter of Mathematics Computing Sciences and Cognition Federal University of ABC
dc.description.affiliationBiotechnology Institute (IBTEC) São Paulo State University (UNESP)
dc.description.affiliationDepartment of Chemical and Biological Sciences São Paulo State University (UNESP)
dc.description.affiliationDepartment of Clinical and Toxicological Analysis Federal University of Ceará
dc.description.affiliationDrug Research and Development Center Federal University of Ceará (UFC)
dc.description.affiliationDepartment of Physiology and Pharmacology School of Medicine Federal University of Ceará (UFC)
dc.description.affiliationGraduate Program in Research and Development Medical Biotechnology Botucatu Medical School (FMB) São Paulo State University (UNESP)
dc.description.affiliationUnespGraduate Program in Tropical Diseases Botucatu Medical School (FMB) São Paulo State University (UNESP)
dc.description.affiliationUnespCenter for the Study of Venoms and Venomous Animals (CEVAP) São Paulo State University (UNESP)
dc.description.affiliationUnespBiotechnology Institute (IBTEC) São Paulo State University (UNESP)
dc.description.affiliationUnespDepartment of Chemical and Biological Sciences São Paulo State University (UNESP)
dc.description.affiliationUnespGraduate Program in Research and Development Medical Biotechnology Botucatu Medical School (FMB) São Paulo State University (UNESP)
dc.identifierhttp://dx.doi.org/10.3390/toxins14090613
dc.identifier.citationToxins, v. 14, n. 9, 2022.
dc.identifier.doi10.3390/toxins14090613
dc.identifier.issn2072-6651
dc.identifier.scopus2-s2.0-85138459485
dc.identifier.urihttp://hdl.handle.net/11449/247641
dc.language.isoeng
dc.relation.ispartofToxins
dc.sourceScopus
dc.subjectBothrops atrox
dc.subjectedema
dc.subjectinflammation
dc.subjectproteomic analysis
dc.subjectsnake venom
dc.subjectthromboinflammation
dc.titleExperimental Bothrops atrox Envenomation: Blood Plasma Proteome Effects after Local Tissue Damage and Perspectives on Thromboinflammationen
dc.typeArtigo
unesp.author.orcid0000-0003-4562-192X[5]
unesp.author.orcid0000-0003-0630-1499[8]
unesp.author.orcid0000-0001-5832-1825[10]

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Artigos - Doenças Tropicais e Diagnósticos por Imagem - FMB
Botucatu - CEVAP - Centro de Estudos de Venenos e Animais Peçonhentos