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Bacterial cellulose-hydroxyapatite composites with osteogenic growth peptide (OGP) or pentapeptide OGP on bone regeneration in critical-size calvarial defect model

dc.contributor.authorPigossi, Suzane C. [UNESP]
dc.contributor.authorOliveira, Guilherme J. P. L. [UNESP]
dc.contributor.authorFinoti, Livia S. [UNESP]
dc.contributor.authorNepomuceno, Rafael [UNESP]
dc.contributor.authorSpolidorio, Luis Carlos [UNESP]
dc.contributor.authorRossa Júnior, Carlos [UNESP]
dc.contributor.authorRibeiro, Sidney J. L. [UNESP]
dc.contributor.authorSaska, Sybele
dc.contributor.authorScarel-Caminaga, Raquel M. [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2015-08-06T16:12:52Z
dc.date.available2015-08-06T16:12:52Z
dc.date.issued2015
dc.description.abstractThis study aimed to evaluate the potential of bacterial cellulose-hydroxyapatite (BC-HA) composites associated with osteogenic growth peptide (OGP) or pentapeptide OGP(10–14) in bone regeneration in critical-size calvarial defects in mice. In this study, the BC-HA, BC-HA-OGP, and BC-HA-OGP(10–14) membranes were analyzed at 3, 7, 15, 30, 60, and 90 days. In each period, the specimens were evaluated by micro-computed tomography (µCT), descriptive histology, gene expression of bone biomarkers by qPCR and VEGFR-2 (vascular endothelial growth factor) quantification by ELISA. Three days post-operative, Runx2, Tnfrsf11b and Bglap bone biomarkers were upregulated mainly by BC-HA OGP and BC-HA OGP(10–14) membranes, suggesting an acceleration of the osteoblast differentiation/activity with the use of these biomaterials. At 60 and 90 days, a high percentage of bone formation was observed by µCT for BC-HA and BC-HA OGP(10–14) membranes. High expression of some bone biomarkers, such as Alpl, Spp1, and Tnfrsf11b, was also observed for the same membranes on days 60 and 90. In conclusion, the BC-HA membrane promoted a better bone formation in critical-size mice calvarial defects. Nevertheless, incorporation of the peptides at the concentration of 10−9 mol L−1 did not improve bone regeneration potential in the long-term.en
dc.description.affiliationUniversidade Estadual Paulista Júlio de Mesquita Filho, Departamento de Diagnóstico e Cirurgia, Faculdade de Odontologia de Araraquara, Araraquara, Rua Humaitá, 1680, Centro, CEP 14801903, SP, Brasil
dc.description.affiliationUnespUniversidade Estadual Paulista Júlio de Mesquita Filho, Departamento de Diagnóstico e Cirurgia, Faculdade de Odontologia de Araraquara, Araraquara, Rua Humaitá, 1680, Centro, CEP 14801903, SP, Brasil
dc.description.affiliationUnespDepartment of Oral Diagnosis and Surgery, School of Dentistry, UNESP—Univ, Estadual Paulista, Araraquara, SP, Brazil
dc.description.affiliationUnespDepartment of Morphology, School of Dentistry, UNESP—Univ, Estadual Paulista, Araraquara, SP, Brazil
dc.description.affiliationUnespDepartment of Physiology and Pathology, School of Dentistry, UNESP—Univ, Estadual Paulista, Araraquara, SP, Brazil
dc.description.affiliationUnespDepartment of General and Inorganic Chemistry, Institute of Chemistry, UNESP—Univ, Estadual Paulista, Araraquara, SP, Brazil
dc.identifierhttp://onlinelibrary.wiley.com/doi/10.1002/jbm.a.35472/abstract
dc.identifier.citationJournal of Biomedical Materials Research, 2015.
dc.identifier.doi10.1002/jbm.a.35472
dc.identifier.issn1549-3296
dc.identifier.lattes2640929291808415
dc.identifier.lattes7634063102292261
dc.identifier.urihttp://hdl.handle.net/11449/125701
dc.language.isoeng
dc.relation.ispartofJournal of Biomedical Materials Research. Part A
dc.relation.ispartofjcr3.231
dc.relation.ispartofsjr0,933
dc.rights.accessRightsAcesso restrito
dc.sourceCurrículo Lattes
dc.subjectBiomaterialen
dc.subjectBacterial celluloseen
dc.subjectBone regenerationen
dc.subjectOsteogenic peptideen
dc.subjectGene expressionen
dc.titleBacterial cellulose-hydroxyapatite composites with osteogenic growth peptide (OGP) or pentapeptide OGP on bone regeneration in critical-size calvarial defect modelen
dc.typeArtigo
unesp.author.lattes2640929291808415
unesp.author.lattes7634063102292261
unesp.author.lattes7634063102292261[6]
unesp.author.orcid0000-0003-3286-9440[7]
unesp.author.orcid0000-0003-1705-5481[6]
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Odontologia, Araraquarapt
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Química, Araraquarapt
unesp.departmentDiagnóstico e Cirurgia - FOARpt
unesp.departmentFisiologia e Patologia - FOARpt
unesp.departmentMorfologia - FOARpt
unesp.departmentQuímica Inorgânica - IQARpt

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