Alchornea glandulosa Ethyl Acetate Fraction Exhibits Antiangiogenic Activity: Preliminary Findings from In Vitro Assays Using Human Umbilical Vein Endothelial Cells

Página do item simplificado
dc.contributor.authorMascia Lopes, Flavia Cristine
dc.contributor.authorRocha, Ana
dc.contributor.authorPirraco, Ana
dc.contributor.authorRegasini, Luis O. [UNESP]
dc.contributor.authorSiqueira, Janaina R. [UNESP]
dc.contributor.authorSilva, Dulce Helena Siqueira [UNESP]
dc.contributor.authorBolzani, Vanderlan da Silva [UNESP]
dc.contributor.authorCarlos, Iracilda Z. [UNESP]
dc.contributor.authorSoares, Raquel
dc.contributor.institutionUniversidade Estadual de Campinas (UNICAMP)
dc.contributor.institutionUniv Porto
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-20T14:21:09Z
dc.date.available2014-05-20T14:21:09Z
dc.date.issued2011-10-01
dc.description.abstractAlchornea glandulosa has traditionally been used in Brazilian folk medicine for the treatment of immune-inflammatory diseases and as an antiulcer agent to heal gastric ulcer and gastritis. Angiogenesis is a complex multistep process that consists of proliferation, migration, and anastomosis of endothelial cells and has a major role in the development of pathologic conditions, such as inflammatory diseases. To investigate a possible link between the anti-inflammatory activities and antiangiogenic effects of A. glandulosa ethyl acetate fraction (AGF), this study examined which features of the angiogenic process could be disturbed by this fraction. The antiangiogenic activity of AGF was determined in vitro by using human umbilical vein endothelial cells (HUVEC), and cell viability, proliferation, apoptosis, invasion, and capillary-like structures formation were addressed. To elucidate the mechanism of action, nuclear factor kappa B (NF kappa B), a transcription factor implicated in these processes, was also evaluated in HUVEC incubated with AGF. A significant decrease in proliferation, a relevant increase in apoptosis, and a strong reduction in invasion capacity (as assessed by bromodeoxyuridine, terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick-end labeling, and double-chamber assays, respectively) were observed. AGF also led to a drastic reduction in the number of capillary-like structures formed when HUVEC were cultured on growth factor-reduced Matrigel-coated plates. In addition, incubation of HUVEC with AGF resulted in reduced NF kappa B activity. These findings emphasize the antiangiogenic potential of AGF and support its therapeutic use for disorders that involve excessive angiogenesis, such as chronic inflammation and tumor growth.en
dc.description.affiliationUniv Campinas UNCAMP, Hematol & Hemotherapy Ctr, Sch Med Sci, São Paulo, Brazil
dc.description.affiliationUniv Porto, Fac Med, Dept Biochem FCT U38, P-4100 Oporto, Portugal
dc.description.affiliationSão Paulo State Univ UNESP, Araraquara Inst Chem, Dept Organ Chem, São Paulo, Brazil
dc.description.affiliationSão Paulo State Univ UNESP, Fac Pharmaceut Sci Araraquara, Dept Clin Anal, São Paulo, Brazil
dc.description.affiliationUnespSão Paulo State Univ UNESP, Araraquara Inst Chem, Dept Organ Chem, São Paulo, Brazil
dc.description.affiliationUnespSão Paulo State Univ UNESP, Fac Pharmaceut Sci Araraquara, Dept Clin Anal, São Paulo, Brazil
dc.description.sponsorshipFCT
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipERAB
dc.description.sponsorshipEuropean Advisory Board
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIdCAPES: 1008/07-2
dc.description.sponsorshipIdEuropean Advisory Board: EA0641
dc.description.sponsorshipIdFAPESP: 03/02176-7
dc.format.extent1244-1253
dc.identifierhttp://dx.doi.org/10.1089/jmf.2010.0204
dc.identifier.citationJournal of Medicinal Food. New Rochelle: Mary Ann Liebert Inc., v. 14, n. 10, p. 1244-1253, 2011.
dc.identifier.doi10.1089/jmf.2010.0204
dc.identifier.fileWOS000295950000021.pdf
dc.identifier.issn1096-620X
dc.identifier.lattes4702004904231248
dc.identifier.lattes4484083685251673
dc.identifier.orcid0000-0002-1516-7765
dc.identifier.urihttp://hdl.handle.net/11449/26325
dc.identifier.wosWOS:000295950000021
dc.language.isoeng
dc.publisherMary Ann Liebert, Inc.
dc.relation.ispartofJournal of Medicinal Food
dc.relation.ispartofjcr1.954
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectAlchornea glandulosaen
dc.subjectantiangiogenicen
dc.subjectethyl acetate fractionen
dc.subjecthuman umbilical vein endothelial cellsen
dc.subjectnuclear factor kappa Ben
dc.titleAlchornea glandulosa Ethyl Acetate Fraction Exhibits Antiangiogenic Activity: Preliminary Findings from In Vitro Assays Using Human Umbilical Vein Endothelial Cellsen
dc.typeArtigo
dcterms.licensehttp://www.liebertpub.com/reprints/journal-of-medicinal-food/38/
dcterms.rightsHolderMary Ann Liebert Inc.
unesp.author.lattes4702004904231248[6]
unesp.author.lattes4484083685251673
unesp.author.orcid0000-0002-1516-7765[6]
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Química, Araraquarapt
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Ciências Farmacêuticas, Araraquarapt

Arquivos

Pacote Original
Agora exibindo 1 - 1 de 1
Imagem de Miniatura
Nome:
WOS000295950000021.pdf
Tamanho:
319.45 KB
Formato:
Adobe Portable Document Format
Baixar
Licença do Pacote
Agora exibindo 1 - 2 de 2
Nenhuma Miniatura disponível
Nome:
license.txt
Tamanho:
1.71 KB
Formato:
Item-specific license agreed upon to submission
Descrição:
Nenhuma Miniatura disponível
Nome:
license.txt
Tamanho:
1.71 KB
Formato:
Item-specific license agreed upon to submission
Descrição:

Coleções

Artigos - Química Orgânica - IQ
Artigos - Análises Clínicas - FCFAR