Rainbow trout slow myoblast cell culture as a model to study slow skeletal muscle, and the characterization of mir-133 and mir-499 families as a case study
dc.contributor.author | da Silva Duran, Bruno Oliveira [UNESP] | |
dc.contributor.author | Dal-Pai-Silva, Maeli [UNESP] | |
dc.contributor.author | de la Serrana, Daniel Garcia | |
dc.contributor.institution | Universidade Estadual Paulista (Unesp) | |
dc.contributor.institution | School of Biology | |
dc.contributor.institution | Physiology and Immunology | |
dc.date.accessioned | 2020-12-12T01:13:39Z | |
dc.date.available | 2020-12-12T01:13:39Z | |
dc.date.issued | 2020-01-01 | |
dc.description.abstract | Muscle fibres are classified as fast, intermediate and slow. In vitro myoblast cell culture model from fast muscle is a very useful tool to study muscle growth and development; however, similar models for slow muscle do not exist. Owing to the compartmentalization of fish muscle fibres, we have developed a slow myoblast cell culture for rainbow trout (Oncorhynchus mykiss). Slow and fast muscle-derived myoblasts have similar morphology, but with differential expression of slow muscle markers such as slow myhc, sox6 and pgc-1α. We also characterized the mir-133 and mir-499 microRNA families in trout slow and fast myoblasts as a case study during myogenesis and in response to electrostimulation. Three mir-133 (a-1a, a-1b and a-2) and four mir-499 (aa, ab, ba and bb) paralogues were identified for rainbow trout and named base on their phylogenetic relationship to zebrafish and Atlantic salmon orthologues. Omy-mir-499ab and omymir-499bb had 0.6 and 0.5-fold higher expression in slow myoblasts compared with fast myoblasts, whereas mir-133 duplicates had similar levels in both phenotypes and little variation during development. Slow myoblasts also showed increased expression for omy-mir-499b paralogues in response to chronic electrostimulation (7-fold increase for omy-mir-499ba and 2.5-fold increase for omy-mir-499bb). The higher expression of mir-499 paralogues in slow myoblasts suggests a role in phenotype determination, while the lack of significant differences of mir-133 copies during culture development might indicate a different role in fish compared with mammals. We have also found signs of sub-functionalization of mir-499 paralogues after electrostimulation, with omy-mir-499b copies more responsive to electrical signals. | en |
dc.description.affiliation | Saõ Paulo State University (UNESP) Institute of Biosciences Department of Morphology | |
dc.description.affiliation | University of St.Andrews Scottish Oceans Institute School of Biology | |
dc.description.affiliation | University of Barcelona Faculty of Biology Department of Cell Biology Physiology and Immunology | |
dc.description.affiliationUnesp | Saõ Paulo State University (UNESP) Institute of Biosciences Department of Morphology | |
dc.description.sponsorship | Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) | |
dc.description.sponsorship | Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) | |
dc.description.sponsorship | Scottish Funding Council | |
dc.description.sponsorshipId | FAPESP: 2015/03234-8 | |
dc.description.sponsorshipId | FAPESP: 2016/05009-4 | |
dc.description.sponsorshipId | FAPESP: 2016/19683-9 | |
dc.description.sponsorshipId | FAPESP: 2019/01592-5 | |
dc.description.sponsorshipId | CNPq: 302656/2015-4 | |
dc.description.sponsorshipId | CNPq: 447233/2014 | |
dc.description.sponsorshipId | Scottish Funding Council: HR09011 | |
dc.identifier | http://dx.doi.org/10.1242/jeb.216390 | |
dc.identifier.citation | Journal of Experimental Biology, v. 223, n. 2, 2020. | |
dc.identifier.doi | 10.1242/jeb.216390 | |
dc.identifier.issn | 0022-0949 | |
dc.identifier.scopus | 2-s2.0-85078692026 | |
dc.identifier.uri | http://hdl.handle.net/11449/198465 | |
dc.language.iso | eng | |
dc.relation.ispartof | Journal of Experimental Biology | |
dc.source | Scopus | |
dc.subject | Cell culture | |
dc.subject | Electrostimulation | |
dc.subject | MiRNA | |
dc.subject | Myoblasts | |
dc.subject | Slow skeletal muscle | |
dc.title | Rainbow trout slow myoblast cell culture as a model to study slow skeletal muscle, and the characterization of mir-133 and mir-499 families as a case study | en |
dc.type | Artigo | |
unesp.author.orcid | 0000-0001-7269-9197[2] |