Immediate human pulp response to ethanol-wet bonding technique

dc.contributor.authorScheffel, Débora Lopes Salles [UNESP]
dc.contributor.authorSacono, Nancy Tomoko
dc.contributor.authorRibeiro, Ana Paula Dias [UNESP]
dc.contributor.authorSoares, Diana Gabriela [UNESP]
dc.contributor.authorBasso, Fernanda Gonçalves [UNESP]
dc.contributor.authorPashley, David
dc.contributor.authorCosta, Carlos Alberto de Souza [UNESP]
dc.contributor.authorHebling, Josimeri [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.description.abstractTo evaluate the short-term response of human pulps to ethanol-wet bonding technique. Methods Deep class V cavities were prepared on 17 sound premolars and divided into three groups. After acid-etching, the cavities from groups 1 (G1) and 2 (G2) were filled with 100% ethanol or distilled water, respectively, for 60 s before the application of Single Bond 2. In group 3 (G3, control), the cavity floor was lined with calcium hydroxide before etching and bonding. All cavities were restored with resin composite. Two teeth were used as intact control. The teeth were extracted 48 h after the clinical procedures. From each tooth serial sections were obtained and stained with haematoxylin and eosin (H/E) and Masson's trichrome. Bacteria microleakage was assessed using Brown & Brenn. All sections were blindly evaluated for five histological features. Results Mean remaining dentine thickness was 463 ± 65 μm (G1); 425 ± 184 μm (G2); and 348 ± 194 μm (G3). Similar pulp reactions followed ethanol- or water-wet bonding techniques. Slight inflammatory responses and disruption of the odontoblast layer related to the cavity floor were seen in all groups. Stained bacteria were not detected in any cavities. Normal pulp tissue was observed in G3 except for one case. Conclusions After 48 h, ethanol-wet bonding does not increase pulpal damage compared to water-wet bonding technique. Clinical significance Ethanol-wet bonding may increase resin-dentine bond durability. This study reported the in vivo response of human pulp tissue when 100% ethanol was applied previously to an etch-and-rinse simplified adhesive system.en
dc.description.affiliationDepartment of Stomatology (Oral Pathology), Dental School, Federal University of Goiás, Av. Décima Primeira Avenida, 62000 – Setor Leste Universitário, Goiânia, Goiás 74605-020, Brazil
dc.description.affiliationDepartment of Dentistry, Dental School, Federal University of Brasilia, Campus Universitário Darcy Ribeiro, Brasília, Distrito Federal 70910-900, Brazil
dc.description.affiliationDepartment of Oral Biology, College of Dental Medicine, Georgia Regents University, 1120 15th Street, CL-2112, Augusta, GA 30912-1129, USA
dc.description.affiliationUnespUniversidade Estadual Paulista Júlio de Mesquita Filho, Departamento de Clínica Infantil, Faculdade de Odontologia de Araraquara, Araraquara, Rua Humaitá, 1680, Centro, CEP 14801-903, SP, Brasil
dc.description.affiliationUnespDepartment of Physiology and Pathology, Araraquara School of Dentistry, UNESP – Univ Estadual Paulista, Rua Humaitá, 1680, Araraquara, São Paulo 14801-903, Brazil
dc.identifier.citationJournal of Dentistry, v. 43, n. 5, p. 537-545, 2015.
dc.relation.ispartofJournal of Dentistry
dc.rights.accessRightsAcesso restrito
dc.sourceCurrículo Lattes
dc.subjectDentine bonding agenten
dc.subjectDental pulpen
dc.titleImmediate human pulp response to ethanol-wet bonding techniqueen
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Odontologia, Araraquarapt
unesp.departmentClínica Infantilpt