Evaluation of in vitro cytotoxic and genotoxic effects of the 3-(3,4-dihydroxyphenyl)-8-hydroxycoumarin

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dc.contributor.authordos Santos Silva, Edvannia
dc.contributor.authorMatos, Maria João
dc.contributor.authorMaistro, Edson Luis [UNESP]
dc.contributor.institutionFAMEMA
dc.contributor.institutionUniversidade de Santiago de Compostela
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.date.accessioned2023-07-29T13:53:50Z
dc.date.available2023-07-29T13:53:50Z
dc.date.issued2023-01-01
dc.description.abstractA wide variety of natural and synthetic coumarins present therapeutic potential. Therefore, the assessment of their safety for humans is essential. 3-(3,4-Dihydroxyphenyl)-8-hydroxycoumarin is a coumarin derivative with antioxidant properties, among other biological activities. The aim of this study is to evaluate the cytotoxic and genotoxic potential of this molecule on peripheral blood mononuclear cells (PBMC) and human hepatocellular carcinoma cells (HepG2/C3A). The results obtained for the cytotoxicity assays, evaluated by the trypan blue staining assay, using concentrations between 0.1 and 20 μg/mL, showed that there is no decrease in cell viability for both cell lines. The MTT assay showed a significant decrease in the viability of HepG2/C3A cells at the highest concentrations tested, after 48 h, for all the tested concentrations, after 72 h of exposure. Regarding the genotoxic assays, the data obtained by the comet assay and the micronucleus test, up to the tested concentration of 10 μg/mL, do not show significant DNA damage and/or chromosomal mutations, for both cell lines. However, at the highest tested concentration of 20 μg/mL, a small but significant genotoxic effect was observed in PBMC. In view of the observed results, it can be concluded that the 3-(3,4-dihydroxyphenyl)-8-hydroxycoumarin, up to a concentration of 10 μg/mL, does not present genotoxic effects in human cells with and without liver enzymes metabolism. Additional studies with higher concentrations of this molecule need to be performed to address its complete biosafety.en
dc.description.affiliationFaculdade de Medicina de Marilia FAMEMA
dc.description.affiliationDepartamento de Química Orgánica Facultade de Farmacia Universidade de Santiago de Compostela
dc.description.affiliationFaculdade de Filosofia e Ciências Departamento de Fonoaudiologia Universidade Estadual Paulista–UNESP
dc.description.affiliationUnespFaculdade de Filosofia e Ciências Departamento de Fonoaudiologia Universidade Estadual Paulista–UNESP
dc.identifierhttp://dx.doi.org/10.1002/jat.4479
dc.identifier.citationJournal of Applied Toxicology.
dc.identifier.doi10.1002/jat.4479
dc.identifier.issn1099-1263
dc.identifier.issn0260-437X
dc.identifier.scopus2-s2.0-85158162916
dc.identifier.urihttp://hdl.handle.net/11449/248792
dc.language.isoeng
dc.relation.ispartofJournal of Applied Toxicology
dc.sourceScopus
dc.subject3-(3′,4′-dihydroxyphenyl)-8-hydroxycoumarin
dc.subjectcomet assay
dc.subjectcytotoxicity
dc.subjectgenotoxicity
dc.subjectmicronucleus assay
dc.titleEvaluation of in vitro cytotoxic and genotoxic effects of the 3-(3,4-dihydroxyphenyl)-8-hydroxycoumarinen
dc.typeArtigo
unesp.author.orcid0000-0003-0757-7876[3]
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Filosofia e Ciências, Maríliapt
unesp.departmentFonoaudiologia - FFCpt

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