Conjugated linoleic acid supplementation changes prostaglandin concentration ratio and alters the expression of genes involved in maternal-fetal recognition from bovine trophoblast cells in vitro

dc.contributor.authorBueno Cordeiro Maldonado, Mariângela [UNESP]
dc.contributor.authorde Castro Lourenço, Valeska [UNESP]
dc.contributor.authorde Oliveira Bezerra, Lucas [UNESP]
dc.contributor.authorFeltrin, Isabella Rio [UNESP]
dc.contributor.authorMendes, Adriano Felipe [UNESP]
dc.contributor.authorRocha, Cecilia Constantino
dc.contributor.authorPugliesi, Guilherme
dc.contributor.authorEaly, Alan D.
dc.contributor.authorMembrive, Claudia Maria Bertan [UNESP]
dc.contributor.authorNogueira, Marcelo Fábio Gouveia [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.contributor.institutionUniversity of Florida (UF-ANS)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionVirginia Tech
dc.date.accessioned2023-07-29T16:13:40Z
dc.date.available2023-07-29T16:13:40Z
dc.date.issued2023-08-01
dc.description.abstractEarly embryonic mortality caused by maternal-fetal recognition failure in the three weeks after fertilization represents a major cause of reproductive inefficiency in the cattle industry. Modifying the amounts and ratios of prostaglandin (PG) F2α and PGE2 can benefit the establishment of pregnancy in cattle. Adding conjugated linoleic acid (CLA) to endometrial and fetal cells culture affects PG synthesis, but its effect on bovine trophoblast cells (CT-1) is unknown. The aim of this study was to determine the effects of CLA (a mixture of cis- and trans-9, 11- and −10,12-octadecadienoic acids) on PGE2 and PGF2α synthesis and the expression of transcripts involved with maternal-fetal recognition of bovine trophectoderm. Cultures of CT-1 were exposed to CLA for 24, 48 and 72 h. Transcript abundance was determined by qRT-PCR and hormone profiles were quantified by ELISA. The PGE2 and PGF2α concentrations were reduced in the culture medium of CLA-exposed CT-1 compared to that of unexposed cells. Furthermore, CLA supplementation increased the PGE2:PGF2α ratio in CT-1 and had a quadratic effect (P < 0.05) on the relative expression of MMP9, PTGES2, and PTGER4. The relative expression levels of PTGER4 were reduced (P < 0.05) in CT-1 cultured with 100 μM CLA than in the unsupplemented and 10 μM-CLA groups. Treatment of CT-1 with CLA decreased PGE2 and PGF2α synthesis but a biphasic effect of CLA was observed on the PGE2:PGF2α ratio and relative abundance of transcripts with 10 μM CLA providing maximal improvements in each endpoint. Our data suggest that CLA may influence eicosanoid metabolic process and extracellular matrix remodeling.en
dc.description.affiliationSão Paulo State University (UNESP-FCL), São Paulo
dc.description.affiliationSão Paulo State University (UNESP-FCAT), Dracena, São Paulo
dc.description.affiliationSão Paulo State University (UNESP-IBB), São Paulo
dc.description.affiliationUniversity of Florida (UF-ANS)
dc.description.affiliationUniversity of São Paulo (USP-FMVZ), Pirassununga, São Paulo
dc.description.affiliationSchool of Animal Sciences Virginia Tech, Blacksburg
dc.description.affiliationUnespSão Paulo State University (UNESP-FCL), São Paulo
dc.description.affiliationUnespSão Paulo State University (UNESP-FCAT), Dracena, São Paulo
dc.description.affiliationUnespSão Paulo State University (UNESP-IBB), São Paulo
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIdFAPESP: 2018/24168-1
dc.description.sponsorshipIdFAPESP: 2019/00637-5
dc.format.extent87-95
dc.identifierhttp://dx.doi.org/10.1016/j.theriogenology.2023.04.003
dc.identifier.citationTheriogenology, v. 206, p. 87-95.
dc.identifier.doi10.1016/j.theriogenology.2023.04.003
dc.identifier.issn0093-691X
dc.identifier.scopus2-s2.0-85159167565
dc.identifier.urihttp://hdl.handle.net/11449/249949
dc.language.isoeng
dc.relation.ispartofTheriogenology
dc.sourceScopus
dc.subjectBlastocyst
dc.subjectCattle
dc.subjectFatty acids
dc.subjectLuteolysis
dc.subjectPregnancy
dc.subjectReproduction
dc.subjectTrophectoderm
dc.titleConjugated linoleic acid supplementation changes prostaglandin concentration ratio and alters the expression of genes involved in maternal-fetal recognition from bovine trophoblast cells in vitroen
dc.typeArtigo
unesp.author.orcid0000-0002-4773-9473[1]
unesp.departmentZootecnia - FCATpt
unesp.departmentCiências Biológicas - FCLASpt

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