TNFSF13B rs9514828 gene polymorphism and soluble B cell activating factor levels: Association with apical periodontitis

Página do item simplificado
dc.contributor.authorCruz, Alvaro
dc.contributor.authorGascón, Luis Gerardo
dc.contributor.authorPalafox-Sánchez, Claudia Azucena
dc.contributor.authorFlores-García, Christian
dc.contributor.authorEspinoza-García, Noemí
dc.contributor.authorSagrero-Fabela, Nefertari
dc.contributor.authorCintra, Luciano Tavares Angelo [UNESP]
dc.contributor.authorMejía-Flores, Rocío
dc.contributor.authorSalazar-Camarena, Diana Celeste
dc.contributor.institutionUniversidad de Guadalajara (UDG)
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.date.accessioned2023-07-29T13:34:54Z
dc.date.available2023-07-29T13:34:54Z
dc.date.issued2023-04-01
dc.description.abstractAim: The aim of this case-control study was to evaluate the association between the TNFSF13B rs9514828 (−871 C > T) polymorphism and soluble BAFF (sBAFF) in apical periodontitis (AP) patients. Methodology: Two hundred and sixty one healthy subjects (HS) and 158 patients with AP classified as: 46 acute apical abscess (AAA), 81 primary AP (pAP) and 31 secondary AP (sAP) patients were included. Genomic DNA (gDNA) was extracted from peripheral blood cells according to the salting out method. The TNFSF13B rs9514828 (NC_000013.11:g.108269025C > T) were identified using polymerase chain reaction (PCR) followed by restriction fragment length polymorphisms (RFLP). Serum sBAFF levels were measured by ELISA test. The chi-squared or Fisher's exact test was performed. Odds ratios (OR) and 95% confidence intervals (95% CI) were calculated to evaluate the risk of AP associated with the rs9514828. The Mann–Whitney U test and Kruskal–Wallis analysis were used for non-normally distributed data. Differences were considered significant with a p-value <.05. Results: No differences in the genotype/allele frequencies were shown between HS and patients with AAA. However, the TT genotype (OR = 2.68, 95% CI: 1.10–6.53; p =.025) and T allele (OR = 1.46, 95% CI: 1.00–2.12; p =.045) were associated with increased risk of pAP. In contrast, the minor allele T significantly decreased the risk of sAP (OR = 0.49, 95% CI: 0.024–0.99; p =.043). sBAFF serum levels were increased in AAA and pAP compared with HS (p <.01 and p =.021, respectively). The AAA patients had higher sBAFF serum levels than pAP (p =.034) and sAP (p <.01). Conclusions: These results suggest that the TNFSF13B rs9514828 (−871 C > T) polymorphism is associated with pAP susceptibility and that BAFF is a cytokine that might be involved in acute and chronic AP. The future exploration of the rs9514828 polymorphism in other AP cohorts is recommended.en
dc.description.affiliationPosgrado en Endodoncia Centro Universitario de Ciencias de la Salud Universidad de Guadalajara (UDG)
dc.description.affiliationInstituto de Investigación en Ciencias Biomédicas (IICB) Centro Universitario de Ciencias de la Salud Universidad de Guadalajara (UDG)
dc.description.affiliationLaboratorio de Investigación en Biomateriales Odontológicos Centro Universitario de Ciencias de la Salud Universidad de Guadalajara (UDG)
dc.description.affiliationGrupo de Inmunología Molecular Centro Universitario de Ciencias de la Salud Universidad de Guadalajara (UDG)
dc.description.affiliationDepartment of Preventive and Restorative Dentistry School of Dentistry São Paulo State University (UNESP)
dc.description.affiliationSchool of Dentistry Dental Assistance Center for Disabled Persons (CAOE) of the São Paulo State University (UNESP)
dc.description.affiliationUnespDepartment of Preventive and Restorative Dentistry School of Dentistry São Paulo State University (UNESP)
dc.description.affiliationUnespSchool of Dentistry Dental Assistance Center for Disabled Persons (CAOE) of the São Paulo State University (UNESP)
dc.description.sponsorshipUniversidad de Guadalajara
dc.description.sponsorshipIdUniversidad de Guadalajara: 265180
dc.format.extent419-431
dc.identifierhttp://dx.doi.org/10.1111/iej.13879
dc.identifier.citationInternational Endodontic Journal, v. 56, n. 4, p. 419-431, 2023.
dc.identifier.doi10.1111/iej.13879
dc.identifier.issn1365-2591
dc.identifier.issn0143-2885
dc.identifier.scopus2-s2.0-85145273574
dc.identifier.urihttp://hdl.handle.net/11449/248118
dc.language.isoeng
dc.relation.ispartofInternational Endodontic Journal
dc.sourceScopus
dc.subjectacute apical abscess
dc.subjectapical periodontitis
dc.subjectrs9514828
dc.subjectsBAFF
dc.subjectTNFSF13B polymorphisms
dc.titleTNFSF13B rs9514828 gene polymorphism and soluble B cell activating factor levels: Association with apical periodontitisen
dc.typeArtigo
unesp.author.orcid0000-0002-4591-4362[1]
unesp.author.orcid0000-0003-4849-3337[2]
unesp.author.orcid0000-0003-0836-4186[3]
unesp.author.orcid0000-0003-2348-7846[7]
unesp.author.orcid0000-0001-7814-0191[9]

Arquivos

Coleções

Araçatuba - CAOE - Centro de Assistência Odontológica a Excepcionais